EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.13 | expressed in Escherichia coli BL21(DE3) cells | Mus musculus |
2.3.1.13 | expression in Escherichia coli | Mus musculus |
2.3.1.71 | expression in Escherichia coli | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.13 | ethanolamine | about 37% inhibition at 100 mM | Mus musculus | |
2.3.1.13 | Gly-Gly-Gly | about 37% inhibition at 100 mM | Mus musculus | |
2.3.1.13 | glycolic acid | - |
Mus musculus | |
2.3.1.13 | additional information | not inhibited by alanine, beta-alanine, valine, and threonine | Mus musculus | |
2.3.1.13 | oleoyl-CoA | - |
Mus musculus | |
2.3.1.13 | serine | about 15% inhibition at 100 mM | Mus musculus | |
2.3.1.13 | tyramine | about 53% inhibition at 100 mM | Mus musculus | |
2.3.1.71 | glycolic acid | - |
Mus musculus | |
2.3.1.71 | oleoyl-CoA | - |
Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.13 | 0.0094 | - |
benzoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 0.0094 | - |
benzoyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 0.045 | - |
hexanoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 0.052 | - |
butyryl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 0.052 | - |
butyryl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 0.209 | - |
acetyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 0.209 | - |
acetyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 6.1 | - |
glycine | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 9.7 | - |
glycine ethyl ester | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 29 | - |
Glycine methyl ester | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 450 | - |
hexanoyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.71 | 0.0094 | - |
benzoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.71 | 6.1 | - |
glycine | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.71 | 9.7 | - |
glycine ethyl ester | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.71 | 29 | - |
Glycine methyl ester | pH 8.0, temperature not specified in the publication | Mus musculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.13 | 34000 | - |
x * 34000, SDS-PAGE | Mus musculus |
2.3.1.13 | 34000 | - |
x * 34000, calculated | Mus musculus |
2.3.1.71 | 34000 | - |
x * 34000, calculated | Mus musculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.13 | Mus musculus | Q91XE0 | - |
- |
2.3.1.71 | Mus musculus | Q91XE0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.13 | nickel affinity column chromatography, and gel filtration | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.13 | acetyl-CoA + glycine | - |
Mus musculus | CoA + N-acetylglycine | - |
? | |
2.3.1.13 | benzoyl-CoA + glycine | best substrate combination | Mus musculus | CoA + N-benzoylglycine | i.e. hippurate | ? | |
2.3.1.13 | benzoyl-CoA + glycine | glycine serves as the best amino donor substrate, and benzoyl-CoA serves as the best the amino acceptor substrate | Mus musculus | CoA + N-benzoylglycine | - |
? | |
2.3.1.13 | benzoyl-CoA + glycine ethyl ester | - |
Mus musculus | CoA + N-benzoylglycine ethyl ester | - |
? | |
2.3.1.13 | benzoyl-CoA + glycine methyl ester | - |
Mus musculus | CoA + N-benzoylglycine methyl ester | - |
? | |
2.3.1.13 | butyryl-CoA + glycine | - |
Mus musculus | CoA + N-butyrylglycine | - |
? | |
2.3.1.13 | hexanoyl-CoA + glycine | - |
Mus musculus | CoA + N-hexanoylglycine | - |
? | |
2.3.1.13 | additional information | enzyme also shows glycine N-benzoyltransferase activity, reaction of EC 2.3.1.71. Amino acceptor substrate preference in decreasing order is benzoyl-CoA, butyryl-CoA, hexanoyl-CoA, acetyl-CoA | Mus musculus | ? | - |
? | |
2.3.1.13 | additional information | no activity with oleoyl-CoA | Mus musculus | ? | - |
? | |
2.3.1.71 | benzoyl-CoA + glycine | glycine serves as the best amino donor substrate, and benzoyl-CoA serves as the best the amino acceptor substrate | Mus musculus | CoA + N-benzoylglycine | - |
? | |
2.3.1.71 | benzoyl-CoA + glycine ethyl ester | - |
Mus musculus | CoA + N-benzoylglycine ethyl ester | - |
? | |
2.3.1.71 | benzoyl-CoA + glycine methyl ester | - |
Mus musculus | CoA + N-benzoylglycine methyl ester | - |
? | |
2.3.1.71 | additional information | enzyme also shows glycine N-acyltransferase activity, reaction of EC 2.3.1.13. Amino acceptor substrate preference in decreasing order is benzoyl-CoA, butyryl-CoA, hexanoyl-CoA, acetyl-CoA | Mus musculus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.13 | ? | x * 34000, SDS-PAGE | Mus musculus |
2.3.1.13 | ? | x * 34000, calculated | Mus musculus |
2.3.1.71 | ? | x * 34000, calculated | Mus musculus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.13 | GlyAT | - |
Mus musculus |
2.3.1.71 | GlyAT | - |
Mus musculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.13 | 0.57 | - |
glycine ethyl ester | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 0.8 | - |
acetyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 0.8 | - |
acetyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 1 | - |
Glycine methyl ester | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 3.2 | - |
glycine | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 4.2 | - |
benzoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 4.2 | - |
benzoyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 4.6 | - |
hexanoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 4.6 | - |
hexanoyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 15 | - |
butyryl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 15 | - |
butyryl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.71 | 0.57 | - |
glycine ethyl ester | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.71 | 1 | - |
Glycine methyl ester | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.71 | 3.2 | - |
glycine | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.71 | 4.2 | - |
benzoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.3.1.13 | 0.021 | - |
at pH 8.0, temperature not specified in the publication | Mus musculus | oleoyl-CoA | |
2.3.1.13 | 58 | - |
apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | glycolic acid | |
2.3.1.71 | 0.021 | - |
pH 8.0, temperature not specified in the publication | Mus musculus | oleoyl-CoA | |
2.3.1.71 | 58 | - |
pH 8.0, temperature not specified in the publication | Mus musculus | glycolic acid |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.13 | 0.035 | - |
Glycine methyl ester | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 0.059 | - |
glycine ethyl ester | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 0.52 | - |
glycine | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 3.8 | - |
acetyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 3.8 | - |
acetyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 100 | - |
hexanoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 100 | - |
hexanoyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 290 | - |
butyryl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 290 | - |
butyryl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 450 | - |
benzoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.13 | 450 | - |
benzoyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.71 | 0.035 | - |
Glycine methyl ester | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.71 | 0.059 | - |
glycine ethyl ester | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.71 | 0.52 | - |
glycine | pH 8.0, temperature not specified in the publication | Mus musculus | |
2.3.1.71 | 450 | - |
benzoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus |