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Literature summary extracted from

  • Dempsey, D.R.; Bond, J.D.; Carpenter, A.M.; Rodriguez Ospina, S.; Merkler, D.J.
    Expression, purification, and characterization of mouse glycine N-acyltransferase in Escherichia coli (2014), Protein Expr. Purif., 97, 23-28.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.13 expressed in Escherichia coli BL21(DE3) cells Mus musculus
2.3.1.13 expression in Escherichia coli Mus musculus
2.3.1.71 expression in Escherichia coli Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.13 ethanolamine about 37% inhibition at 100 mM Mus musculus
2.3.1.13 Gly-Gly-Gly about 37% inhibition at 100 mM Mus musculus
2.3.1.13 glycolic acid
-
Mus musculus
2.3.1.13 additional information not inhibited by alanine, beta-alanine, valine, and threonine Mus musculus
2.3.1.13 oleoyl-CoA
-
Mus musculus
2.3.1.13 serine about 15% inhibition at 100 mM Mus musculus
2.3.1.13 tyramine about 53% inhibition at 100 mM Mus musculus
2.3.1.71 glycolic acid
-
Mus musculus
2.3.1.71 oleoyl-CoA
-
Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.13 0.0094
-
benzoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 0.0094
-
benzoyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 0.045
-
hexanoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 0.052
-
butyryl-CoA pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 0.052
-
butyryl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 0.209
-
acetyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 0.209
-
acetyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 6.1
-
glycine apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 9.7
-
glycine ethyl ester apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 29
-
Glycine methyl ester apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 450
-
hexanoyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.71 0.0094
-
benzoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.71 6.1
-
glycine pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.71 9.7
-
glycine ethyl ester pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.71 29
-
Glycine methyl ester pH 8.0, temperature not specified in the publication Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.13 34000
-
x * 34000, SDS-PAGE Mus musculus
2.3.1.13 34000
-
x * 34000, calculated Mus musculus
2.3.1.71 34000
-
x * 34000, calculated Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.13 Mus musculus Q91XE0
-
-
2.3.1.71 Mus musculus Q91XE0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.13 nickel affinity column chromatography, and gel filtration Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.13 acetyl-CoA + glycine
-
Mus musculus CoA + N-acetylglycine
-
?
2.3.1.13 benzoyl-CoA + glycine best substrate combination Mus musculus CoA + N-benzoylglycine i.e. hippurate ?
2.3.1.13 benzoyl-CoA + glycine glycine serves as the best amino donor substrate, and benzoyl-CoA serves as the best the amino acceptor substrate Mus musculus CoA + N-benzoylglycine
-
?
2.3.1.13 benzoyl-CoA + glycine ethyl ester
-
Mus musculus CoA + N-benzoylglycine ethyl ester
-
?
2.3.1.13 benzoyl-CoA + glycine methyl ester
-
Mus musculus CoA + N-benzoylglycine methyl ester
-
?
2.3.1.13 butyryl-CoA + glycine
-
Mus musculus CoA + N-butyrylglycine
-
?
2.3.1.13 hexanoyl-CoA + glycine
-
Mus musculus CoA + N-hexanoylglycine
-
?
2.3.1.13 additional information enzyme also shows glycine N-benzoyltransferase activity, reaction of EC 2.3.1.71. Amino acceptor substrate preference in decreasing order is benzoyl-CoA, butyryl-CoA, hexanoyl-CoA, acetyl-CoA Mus musculus ?
-
?
2.3.1.13 additional information no activity with oleoyl-CoA Mus musculus ?
-
?
2.3.1.71 benzoyl-CoA + glycine glycine serves as the best amino donor substrate, and benzoyl-CoA serves as the best the amino acceptor substrate Mus musculus CoA + N-benzoylglycine
-
?
2.3.1.71 benzoyl-CoA + glycine ethyl ester
-
Mus musculus CoA + N-benzoylglycine ethyl ester
-
?
2.3.1.71 benzoyl-CoA + glycine methyl ester
-
Mus musculus CoA + N-benzoylglycine methyl ester
-
?
2.3.1.71 additional information enzyme also shows glycine N-acyltransferase activity, reaction of EC 2.3.1.13. Amino acceptor substrate preference in decreasing order is benzoyl-CoA, butyryl-CoA, hexanoyl-CoA, acetyl-CoA Mus musculus ?
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.13 ? x * 34000, SDS-PAGE Mus musculus
2.3.1.13 ? x * 34000, calculated Mus musculus
2.3.1.71 ? x * 34000, calculated Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.13 GlyAT
-
Mus musculus
2.3.1.71 GlyAT
-
Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.13 0.57
-
glycine ethyl ester apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 0.8
-
acetyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 0.8
-
acetyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 1
-
Glycine methyl ester apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 3.2
-
glycine apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 4.2
-
benzoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 4.2
-
benzoyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 4.6
-
hexanoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 4.6
-
hexanoyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 15
-
butyryl-CoA pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 15
-
butyryl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.71 0.57
-
glycine ethyl ester pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.71 1
-
Glycine methyl ester pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.71 3.2
-
glycine pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.71 4.2
-
benzoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.13 0.021
-
at pH 8.0, temperature not specified in the publication Mus musculus oleoyl-CoA
2.3.1.13 58
-
apparent value, at pH 8.0, temperature not specified in the publication Mus musculus glycolic acid
2.3.1.71 0.021
-
pH 8.0, temperature not specified in the publication Mus musculus oleoyl-CoA
2.3.1.71 58
-
pH 8.0, temperature not specified in the publication Mus musculus glycolic acid

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.13 0.035
-
Glycine methyl ester apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 0.059
-
glycine ethyl ester apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 0.52
-
glycine apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 3.8
-
acetyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 3.8
-
acetyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 100
-
hexanoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 100
-
hexanoyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 290
-
butyryl-CoA pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 290
-
butyryl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 450
-
benzoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.13 450
-
benzoyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.71 0.035
-
Glycine methyl ester pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.71 0.059
-
glycine ethyl ester pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.71 0.52
-
glycine pH 8.0, temperature not specified in the publication Mus musculus
2.3.1.71 450
-
benzoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus