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Literature summary extracted from

  • Hunter, R.W.; Zeqiraj, E.; Morrice, N.; Sicheri, F.; Sakamoto, K.
    Expression and purification of functional human glycogen synthase-1:glycogenin-1 complex in insect cells (2015), Protein Expr. Purif., 108, 23-29.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.186 gene GYG1, recombinant expression of functional GST-tagged human muscle glycogen synthase in complex with human glycogenin-1 in Spodoptera frugiperda Sf9 cells using a bicistronic pFastBac™-Dual expression vector and baculovirus transfection Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.186 Mg2+ required Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.186 Homo sapiens P46976 gene GYG1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.186 glycoprotein the degree of glycosylation of the enzyme is modest Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.186 recombinant GST-tagged glycogen synthase in complex with glycogenin-1 from Sf9 insect cells by glutathione affinity chromatography and gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.186 skeletal muscle
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.186 UDP-alpha-D-glucose + glycogenin-1 self-glucosylation of the glycosyltransferase glycogenin-1 Homo sapiens UDP + alpha-D-glucosylglycogenin-1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.186 glycogenin-1
-
 Homo sapiens
2.4.1.186 GN1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.186 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.186 7.4
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.1.186 additional information the recombinant glycogen synthase-glycogenin-1 complex GYS1:GN1 is functional and exhibits both allosteric and phospho-dependent regulatio, activation of GYS1:GN1 complex by GYS1 dephosphorylationn Homo sapiens
2.4.1.186 physiological function glycogenin-1 is a constitutively active enzyme so does not represent a point of regulation Homo sapiens