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Literature summary extracted from

  • Romanow, W.G.; Piper, D.E.; Fordstrom, P.; Thibault, S.; Zhou, M.; Walker, N.P.
    BacMam production of active recombinant lecithin-cholesterol acyltransferase: expression, purification and characterization (2016), Protein Expr. Purif., 105, 1-6.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.43 drug development a therapeutic that increases enzyme LCAT activity may promote reverse cholesterol transport and prove beneficial for the treatment of dyslipidaemia and atherosclerosis Homo sapiens
2.3.1.43 medicine a therapeutic that increases enzyme LCAT activity may promote reverse cholesterol transport and prove beneficial for the treatment of dyslipidaemia and atherosclerosis Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.43 gene lcat, use of baculovirus as a transducing vector to express the C-or N-terminally His-tagged enzyme in insect cell line Sf9, and to transiently express tagged LCAT in human HEK-293 GnTI cells, deficient in N-acetylglucosaminyl transferase I and expression of the recombinant protein as a high-mannose glycoform (of the MAN9GlcNAc2 or MAN5GlcNAc2 types) suitable for deglycosylation by Endo H, which leaves a single N-acetyl glucosamine (GlcNAc) residue at the glycosylation sites, to avoid protein aggregation coccuring with fully deglycosylated protein. Or expression in HEK-293 6E cells. TEV or Factor Xa sites are introduced for tag cleavage. The enzyme is secreted. Method development and evaluation, overview Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.43 C31Y site-directed mutagenesis, the mutation renders the enzyme more stable and active than the native form Homo sapiens
2.3.1.43 additional information removal of a proline rich region (residues 402 to 416) on the C-terminus Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.43 extracellular the enzyme is secreted Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.43 49000 62000 recombinant enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.43 phosphatidylcholine + a sterol Homo sapiens
-
 1-acylglycerophosphocholine + a sterol ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.43 Homo sapiens P04180 gene lcat
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.43 glycoprotein the enzyme is highly glycosylated with four potential N-linked glycosylation sites and two putative O-linked sites Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.43 recombinant partially deglycosylated His-tagged enzyme from HEK-293 GnTI cells by nickel affinity chromatography to homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.43 blood plasma
-
 Homo sapiens
-
2.3.1.43 liver produced predominantly in the liver and secreted as a circulating protein into the serum Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.43 phosphatidylcholine + a sterol
-
 Homo sapiens 1-acylglycerophosphocholine + a sterol ester
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.43 LCAT
-
 Homo sapiens
2.3.1.43 lecithin-cholesterol acyltransferase
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.43 evolution LCAT is a member of the alpha/beta hydrolase family Homo sapiens
2.3.1.43 physiological function a key enzyme in the esterification of cholesterol and its subsequent incorporation into the core of high density lipoprotein (HDL) particles. The enzyme is also also involved in reverse cholesterol transport, the mechanism by which cholesterol is removed from peripheral cells and transported to the liver for excretion Homo sapiens