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Literature summary extracted from

  • Wang, Z.; Lin, M.; Wang, L.; Ammar, E.; Yang, S.
    Metabolic engineering of Propionibacterium freudenreichii subsp. shermanii for enhanced propionic acid fermentation: Effects of overexpressing three biotin-dependent carboxylases (2015), Process Biochem., 50, 194-204.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.1 gene mmc, co-overexpression of pyruvate carboxylase, methylmalonyl-CoA decarboxylase, and methylmalonyl-CoA carboxyltransferase from Propionibacterium acidipropionici ATCC 4875 in Propionibacterium shermanii, the MMC activity increases by 107% Propionibacterium freudenreichii

Protein Variants

EC Number Protein Variants Comment Organism
2.1.3.1 additional information metabolic engineering of Propionibacteriumfreudenreichii subsp. shermanii DSM 4902 for enhanced propionic acid fermentation by overexpression of pyruvate carboxylase, methylmalonyl-CoA decarboxylase, and methylmalonyl-CoA carboxyltransferase, three biotin-dependent carboxylases in the dicarboxylic acid pathway controlling the carbon fluxin the Wood–Werkman cycle, from Propionibacterium acidipropionici ATCC 4875 in Propionibacterium shermani. The co-fermentation kinetics show that with more glycerol as carbon source, both the wild-type and Ps(pKPYC4) produce more propionic acid and succinic acid, but acetic acid formation is not as significantly affected, neither is the Gly/Glu ratio, metabolic flux analysis, detailed overview Propionibacterium freudenreichii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.1 (S)-methylmalonyl-CoA + pyruvate Propionibacterium freudenreichii
-
 propanoyl-CoA + oxaloacetate
-
 ?
2.1.3.1 (S)-methylmalonyl-CoA + pyruvate Propionibacterium freudenreichii DSM 4902
-
 propanoyl-CoA + oxaloacetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.1 Propionibacterium freudenreichii
-
 subsp. shermanii, gene mmc
-
2.1.3.1 Propionibacterium freudenreichii DSM 4902
-
 subsp. shermanii, gene mmc
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.1 (S)-methylmalonyl-CoA + pyruvate
-
 Propionibacterium freudenreichii propanoyl-CoA + oxaloacetate
-
 ?
2.1.3.1 (S)-methylmalonyl-CoA + pyruvate
-
 Propionibacterium freudenreichii DSM 4902 propanoyl-CoA + oxaloacetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.3.1 methylmalonyl-CoA carboxyltransferase
-
 Propionibacterium freudenreichii
2.1.3.1 MMC
-
 Propionibacterium freudenreichii

General Information

EC Number General Information Comment Organism
2.1.3.1 metabolism the enzyme is one of three biotin-dependent carboxylases in the dicarboxylic acid pathway controlling the carbon fluxin the Wood-Werkman cycle, metabolic flux analysis Propionibacterium freudenreichii