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Literature summary extracted from

  • Suarez, J.; Schramm, V.L.
    Isotope-specific and amino acid-specific heavy atom substitutions alter barrier crossing in human purine nucleoside phosphorylase (2015), Proc. Natl. Acad. Sci. USA, 112, 11247-11251.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.1 expressed in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.092
-
 guanosine native enzyme, at pH 7.4 and 25°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.1 purine deoxyribonucleoside + phosphate Homo sapiens
-
 purine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 purine ribonucleoside + phosphate Homo sapiens
-
 purine + alpha-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 adenosine + phosphate
-
 Homo sapiens adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate
-
 Homo sapiens guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Homo sapiens hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 purine deoxyribonucleoside + phosphate
-
 Homo sapiens purine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 purine ribonucleoside + phosphate
-
 Homo sapiens purine + alpha-D-ribose 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.1 PNP
-
 Homo sapiens
2.4.2.1 purine nucleoside phosphorylase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 13.4
-
 guanosine native enzyme, at pH 7.4 and 25°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.1 150
-
 guanosine native enzyme, at pH 7.4 and 25°C Homo sapiens