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Literature summary extracted from

  • Zhu, J.G.; Ochalek, J.T.; Kaufmann, M.; Jones, G.; Deluca, H.F.
    CYP2R1 is a major, but not exclusive, contributor to 25-hydroxyvitamin D production in vivo (2013), Proc. Natl. Acad. Sci. USA, 110, 15650-15655.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.24 gene CYP2R1, quantitative real-time PCR enzyme expression analysis Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.24 additional information construction of null mutant enzyme-deficient Cyp2r1-/- mice, that show greater than 50% reduction in serum 25-hydroxyvitamin D3 level, while the 1alpha,25-dihydroxyvitamin D3 level in the serum remains unchanged. A double knockout of Cyp2r1 and Cyp27a1, the enzyme responsible for the 1alpha-hydroxylation step, maintain a similar circulating level of 25-hydroxyvitamin D3 and 1alpha,25-dihydroxyvitamin D3 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.24 calciol + O2 + [reduced NADPH-hemoprotein reductase] Mus musculus
-
calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.24 calciol + O2 + [reduced NADPH-hemoprotein reductase] Mus musculus C57BL/6
-
calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.24 Mus musculus Q6VVW9
-
-
1.14.14.24 Mus musculus C57BL/6 Q6VVW9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.24 liver
-
Mus musculus
-
1.14.14.24 additional information quantitative real-time PCR enzyme expression analysis, no or low expression in kidney and ovaries Mus musculus
-
1.14.14.24 testis
-
Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.24 calciol + O2 + [reduced NADPH-hemoprotein reductase]
-
Mus musculus calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.24 calciol + O2 + [reduced NADPH-hemoprotein reductase]
-
Mus musculus C57BL/6 calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.24 CYP2R1
-
Mus musculus
1.14.14.24 vitamin D 25-hydroxylase
-
Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.24 37
-
assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.24 heme
-
Mus musculus
1.14.14.24 NADPH
-
Mus musculus

General Information

EC Number General Information Comment Organism
1.14.14.24 malfunction enzyme-deficient Cyp2r1-/- mice show greater than 50% reduction in serum 25-hydroxyvitamin D3 level, while the 1alpha,25-dihydroxyvitamin D3 level in the serum remains unchanged. A double knockout of Cyp2r1 and Cyp27a1, the enzyme responsible for the 1alpha-hydroxylation step, maintain a similar circulating level of 25-hydroxyvitamin D3 and 1alpha,25-dihydroxyvitamin D3 Mus musculus
1.14.14.24 physiological function CYP2R1 is a major, but not exclusive, contributor to 25-hydroxyvitamin D production in vivo. A second enzyme is another contributor to this important step in vitamin D activation Mus musculus