EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.24 | gene CYP2R1, quantitative real-time PCR enzyme expression analysis | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.24 | additional information | construction of null mutant enzyme-deficient Cyp2r1-/- mice, that show greater than 50% reduction in serum 25-hydroxyvitamin D3 level, while the 1alpha,25-dihydroxyvitamin D3 level in the serum remains unchanged. A double knockout of Cyp2r1 and Cyp27a1, the enzyme responsible for the 1alpha-hydroxylation step, maintain a similar circulating level of 25-hydroxyvitamin D3 and 1alpha,25-dihydroxyvitamin D3 | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.24 | calciol + O2 + [reduced NADPH-hemoprotein reductase] | Mus musculus | - |
calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.24 | calciol + O2 + [reduced NADPH-hemoprotein reductase] | Mus musculus C57BL/6 | - |
calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.24 | Mus musculus | Q6VVW9 | - |
- |
1.14.14.24 | Mus musculus C57BL/6 | Q6VVW9 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.24 | liver | - |
Mus musculus | - |
1.14.14.24 | additional information | quantitative real-time PCR enzyme expression analysis, no or low expression in kidney and ovaries | Mus musculus | - |
1.14.14.24 | testis | - |
Mus musculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.24 | calciol + O2 + [reduced NADPH-hemoprotein reductase] | - |
Mus musculus | calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.24 | calciol + O2 + [reduced NADPH-hemoprotein reductase] | - |
Mus musculus C57BL/6 | calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.24 | CYP2R1 | - |
Mus musculus |
1.14.14.24 | vitamin D 25-hydroxylase | - |
Mus musculus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.24 | 37 | - |
assay at | Mus musculus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.24 | heme | - |
Mus musculus | |
1.14.14.24 | NADPH | - |
Mus musculus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.24 | malfunction | enzyme-deficient Cyp2r1-/- mice show greater than 50% reduction in serum 25-hydroxyvitamin D3 level, while the 1alpha,25-dihydroxyvitamin D3 level in the serum remains unchanged. A double knockout of Cyp2r1 and Cyp27a1, the enzyme responsible for the 1alpha-hydroxylation step, maintain a similar circulating level of 25-hydroxyvitamin D3 and 1alpha,25-dihydroxyvitamin D3 | Mus musculus |
1.14.14.24 | physiological function | CYP2R1 is a major, but not exclusive, contributor to 25-hydroxyvitamin D production in vivo. A second enzyme is another contributor to this important step in vitamin D activation | Mus musculus |