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Literature summary extracted from

  • Shukla, S.; Verma, A.; Kajala, I.; Nyyssolae, A.; Baruah, R.; Katina, K.; Juvonen, R.; Tenkanen, M.; Goyal, A.
    Structure modelling and functional analysis of recombinant extransucrase from Weissella confusa Cab3 expressed in Lactococcus lactis (2016), Prep. Biochem. Biotechnol., 46, 822-832.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.5 recombinant soluble extracellular enzyme expression in Lactococcus lactis Weissella confusa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.5 6.2
-
 [(1->6)-alpha-D-glucosyl]n pH 5.0, 35°C Weissella confusa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.5 180000
-
 x * 180000, recombinant enzyme, SDS-PAGE Weissella confusa

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Weissella confusa X4QKX0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 recombinant extracellular enzyme 4fold from Lactococcus lactis by PEG fractionation Weissella confusa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.5 1.5
-
 partially purrified recombinant enzyme, pH 5.0, 35°C Weissella confusa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n dextran Weissella confusa D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.5 ? x * 180000, recombinant enzyme, SDS-PAGE Weissella confusa
2.4.1.5 More the enzyme structure contains the domains A, B, C, IV, and V, structure superposition, overview Weissella confusa

Synonyms

EC Number Synonyms Comment Organism
2.4.1.5 Cab3
-
 Weissella confusa
2.4.1.5 DSR
-
 Weissella confusa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 35
-
 recombinant enzyme Weissella confusa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5
-
 recombinant enzyme Weissella confusa

General Information

EC Number General Information Comment Organism
2.4.1.5 additional information structure modeling using the crystal structure of the enzyme from Lactobacillus reuteri, PDB ID 3HZ3, and Streptococcus mutans, PDB ID 3AlB, as templates. Asp510 acts as a nucleophile, Glu548 acts as catalytic acid/base, and Asp621 acts a stransition state stabilizer Weissella confusa