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Literature summary extracted from

  • Mohan Rao, T.J.; Goyal, A.
    Purification, optimization of assay, and stability studies of dextransucrase isolated from Weissella cibaria JAG8 (2013), Prep. Biochem. Biotechnol., 43, 329-341.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.5 EDTA 64% inhibition at 10 mM Weissella cibaria
2.4.1.5 Urea inactivation at 5 M Weissella cibaria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.5 additional information
-
 additional information Michaelis-Menten kinetics Weissella cibaria
2.4.1.5 13
-
 [(1->6)-alpha-D-glucosyl]n pH 5.4, 35°C Weissella cibaria

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.5 Mg2+ activates at 2 mM Weissella cibaria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.5 177000
-
 x * 177000, SDS-PAGE Weissella cibaria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n Weissella cibaria production of dextran D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n Weissella cibaria JAG8 production of dextran D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Weissella cibaria B9UNL6 isolated from apple, gene dsrWC
-
2.4.1.5 Weissella cibaria JAG8 B9UNL6 isolated from apple, gene dsrWC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 native enzyme 37fold by PEG400 fractionation and gel filtration Weissella cibaria

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.5 1
-
 partially purrified recombinant enzyme, pH 5.4, 35°C Weissella cibaria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n production of dextran Weissella cibaria D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n production of dextran, optimal at 5% w/v sucrose Weissella cibaria D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n production of dextran Weissella cibaria JAG8 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n production of dextran, optimal at 5% w/v sucrose Weissella cibaria JAG8 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.5 ? x * 177000, SDS-PAGE Weissella cibaria

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 35
-
-
 Weissella cibaria

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.5 35
-
 purified enzyme, pH 5.4, 10-500 mM sodium acetate buffer, 1 h, stable Weissella cibaria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.4
-
-
 Weissella cibaria

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.5 5.4
-
 purified enzyme, 35°C, 10-500 mM sodium acetate buffer, 1 h, stable Weissella cibaria