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Literature summary extracted from

  • Hyakutake, M.; Tomizawa, S.; Sugahara, I.; Murata, E.; Mizuno, K.; Abe, H.; Tsuge, T.
    Carboxy-terminal modification of polyhydroxyalkanoate (PHA) via alcoholysis reaction catalyzed by class IV PHA synthase (2015), Polym. Degrad. Stab., 117, 90-96.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.3.1.304 synthesis enzyme shows both polyhydroxyalkanoate polymerization and alcoholysis activities, unsing various alcohols other than ethanol for alcoholysis.. Through the use of bifunctional compounds for alcoholysis, the polyhydroxyalkanoate carboxy terminus can be modified with thiol, alkynyl, hydroxy, and benzyl groups, resulting in the functionalization of the polyhydroxyalkanoate carboxy terminus Bacillus cereus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.304 expression in Escherichia coli Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Bacillus cereus D2Z0B8 and D2Z0B6 D2Z0B8 i.e. subunit PhaC, D2Z0B6 i.e. subunit PhaR
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2.3.1.304 Bacillus cereus YB-4 D2Z0B8 and D2Z0B6 D2Z0B8 i.e. subunit PhaC, D2Z0B6 i.e. subunit PhaR
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.304 additional information enzyme shows both PHA polymerization and alcoholysis activities. For alcoholysis, PhaRC utilizes various alcohols other than ethanol for alcoholysis, leading to the PHA carboxy terminus modified with thiol, alkynyl, hydroxy, and benzyl groups Bacillus cereus ?
-
 ?
2.3.1.304 additional information enzyme shows both PHA polymerization and alcoholysis activities. For alcoholysis, PhaRC utilizes various alcohols other than ethanol for alcoholysis, leading to the PHA carboxy terminus modified with thiol, alkynyl, hydroxy, and benzyl groups Bacillus cereus YB-4 ?
-
 ?