BRENDA - Enzyme Database

Ethenoguanines undergo glycosylation by nucleoside 2'-deoxyribosyltransferases at non-natural sites

Ye, W.; Paul, D.; Gao, L.; Seckute, J.; Sangaiah, R.; Jayaraj, K.; Zhang, Z.; Kaminski, P.A.; Ealick, S.E.; Gold, A.; Ball, L.M.; PLoS ONE 9, e115082 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.4.2.6
expressed in Escherichia coli BL21(DE3)pLysS cells
Lactobacillus fermentum
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.4.2.6
Lactobacillus fermentum
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.4.2.6
ammonium sulfate precipitation and Sephacryl S-200 gel filtration
Lactobacillus fermentum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.4.2.6
2'-deoxycytidine + adenosine
-
737137
Lactobacillus fermentum
2'-deoxyadenosine + cytosine
-
-
-
?
2.4.2.6
additional information
the enzyme glycosylates N2,3-ethenoguanine (8,9-dihydro-9-oxoimidazo[2,1-b]purine) at N7 and N1 with somewhat less selectivity for N7
737137
Lactobacillus fermentum
?
-
-
-
-
Synonyms
EC Number
Synonyms
Commentary
Organism
2.4.2.6
NDT
-
Lactobacillus fermentum
2.4.2.6
nucleoside 2'-deoxyribosyltransferase
-
Lactobacillus fermentum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.6
expressed in Escherichia coli BL21(DE3)pLysS cells
Lactobacillus fermentum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.6
ammonium sulfate precipitation and Sephacryl S-200 gel filtration
Lactobacillus fermentum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.4.2.6
2'-deoxycytidine + adenosine
-
737137
Lactobacillus fermentum
2'-deoxyadenosine + cytosine
-
-
-
?
2.4.2.6
additional information
the enzyme glycosylates N2,3-ethenoguanine (8,9-dihydro-9-oxoimidazo[2,1-b]purine) at N7 and N1 with somewhat less selectivity for N7
737137
Lactobacillus fermentum
?
-
-
-
-