BRENDA - Enzyme Database show

Ethenoguanines undergo glycosylation by nucleoside 2'-deoxyribosyltransferases at non-natural sites

Ye, W.; Paul, D.; Gao, L.; Seckute, J.; Sangaiah, R.; Jayaraj, K.; Zhang, Z.; Kaminski, P.A.; Ealick, S.E.; Gold, A.; Ball, L.M.; PLoS ONE 9, e115082 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.4.2.6
expressed in Escherichia coli BL21(DE3)pLysS cells
Lactobacillus fermentum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.6
Lactobacillus fermentum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.6
ammonium sulfate precipitation and Sephacryl S-200 gel filtration
Lactobacillus fermentum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
2'-deoxycytidine + adenosine
-
737137
Lactobacillus fermentum
2'-deoxyadenosine + cytosine
-
-
-
?
2.4.2.6
additional information
the enzyme glycosylates N2,3-ethenoguanine (8,9-dihydro-9-oxoimidazo[2,1-b]purine) at N7 and N1 with somewhat less selectivity for N7
737137
Lactobacillus fermentum
?
-
-
-
-
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.6
expressed in Escherichia coli BL21(DE3)pLysS cells
Lactobacillus fermentum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.6
ammonium sulfate precipitation and Sephacryl S-200 gel filtration
Lactobacillus fermentum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
2'-deoxycytidine + adenosine
-
737137
Lactobacillus fermentum
2'-deoxyadenosine + cytosine
-
-
-
?
2.4.2.6
additional information
the enzyme glycosylates N2,3-ethenoguanine (8,9-dihydro-9-oxoimidazo[2,1-b]purine) at N7 and N1 with somewhat less selectivity for N7
737137
Lactobacillus fermentum
?
-
-
-
-