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Literature summary extracted from

  • Fang, J.Y.; Tan, S.J.; Yang, Z.; Tayag, C.; Han, B.
    Tumor bioengineering using a transglutaminase crosslinked hydrogel (2014), PLoS ONE, 9, e105616.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.13 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.2.13 hanging drop vapor diffusion method, using 20 mM MES at pH 6.8, 200 mM sodium chloride, 20 mM MgCl2, 6% (w/v) PEG 3350, 5 mM dithiothreitol, and 24% (v/v) glycerol Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 GTP
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 78520
-
 1 * 78520, including the C-terminal His-tag and GTP, calculated from amino acid sequence Homo sapiens
2.3.2.13 78570
-
 1 * 78570, multiangle light scattering Homo sapiens
2.3.2.13 80000
-
 1 * 80000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.13 [protein]-L-glutamine + alkylamine Homo sapiens
-
 [protein]-N5-alkyl-L-glutamine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 Ni-NTA affinity resin column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 [protein]-L-glutamine + alkylamine
-
 Homo sapiens [protein]-N5-alkyl-L-glutamine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 monomer 1 * 80000, SDS-PAGE Homo sapiens
2.3.2.13 monomer 1 * 78520, including the C-terminal His-tag and GTP, calculated from amino acid sequence Homo sapiens
2.3.2.13 monomer 1 * 78570, multiangle light scattering Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 TG2
-
 Homo sapiens
2.3.2.13 transglutaminase2
-
 Homo sapiens