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Literature summary extracted from

  • Naganuma, T.; Kihara, A.
    Two modes of regulation of the fatty acid elongase ELOVL6 by the 3-ketoacyl-CoA reductase KAR in the fatty acid elongation cycle (2014), PLoS ONE, 9, e101823.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.199 additional information the 3-ketoacyl-CoA reductase, KAR, the enzyme catalyzing the next step in metabolism after fatty acid elongase ELOVL6, largely stimulates ELOVL6 activity. Activity of purified enzyme ELOVL6 is enhanced by about 3fold in the presence of 3-ketoacyl-CoA reductase, KAR. This effect is KAR enzyme activity-independent, since it is observed in the absence of NADPH and in the poorly active or inactive KAR mutants, overview. ELOVL6 enzyme activity is also enhanced in a KAR enzyme activity-dependent manner Homo sapiens
2.3.1.199 NADPH ELOVL6 activity in membrane fractions is enhanced in the presence of NADPH Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.199 recombinant expression of N-terminally 3xFLAG-tagged enzyme ELOVL6 in HEK-293 T cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.199 0.00122
-
palmitoyl-CoA pH 6.8, 37°C, recombinant enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.199 membrane
-
Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.199 Mg2+ required Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.199 29000
-
x * 29000, recombinant deglycosylated 3xFLAG-tagged enzyme, SDS-PAGE, x * 32000, recombinant glycosylated 3xFLAG-tagged enzyme, SDS-PAGE Homo sapiens
2.3.1.199 32000
-
x * 29000, recombinant deglycosylated 3xFLAG-tagged enzyme, SDS-PAGE, x * 32000, recombinant glycosylated 3xFLAG-tagged enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.199 a very-long-chain acyl-CoA + malonyl-CoA Homo sapiens
-
a very-long-chain 3-oxoacyl-CoA + CO2 + coenzyme A
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.199 Homo sapiens Q9H5J4
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.199 glycoprotein deglycosylation is possibel with Endo H Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.199 recombinant N-terminally 3xFLAG-tagged enzyme ELOVL6 from HEK-293 T cells by solubilization with Triton X-100 and affinity chromatography Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.199 recombinant enzyme ELOVL6 has no enzyme activity under solubilized conditions, reconstitution of ELOVL6 into proteoliposomes enables it to exhibit enzyme activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.199 a very-long-chain acyl-CoA + malonyl-CoA
-
Homo sapiens a very-long-chain 3-oxoacyl-CoA + CO2 + coenzyme A
-
?
2.3.1.199 additional information no activity with saturated and unsaturated fatty acids longer than C16 Homo sapiens ?
-
?
2.3.1.199 palmitoyl-CoA + malonyl-CoA
-
Homo sapiens 3-oxostearoyl-CoA + CO2 + CoA
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.199 ? x * 29000, recombinant deglycosylated 3xFLAG-tagged enzyme, SDS-PAGE, x * 32000, recombinant glycosylated 3xFLAG-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.199 Elovl6
-
Homo sapiens
2.3.1.199 FA elongase
-
Homo sapiens
2.3.1.199 Fatty acid elongase
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.199 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.199 6.8
-
assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.199 metabolism the enzyme is involved in the fatty acid elongation cycle consisting of the reaction steps of condensation, reduction, dehydration, and reduction Homo sapiens
2.3.1.199 physiological function 3-ketoacyl-CoA reductase, KAR, regulates enzyme ELOVL6 via two modes: in the first mode, KAR may induce conformational changes in ELOVL6 to become a structure that can undergo catalysis. In the second mode, conversion of 3-oxoacyl-CoA to 3-hydroxyacyl-CoA by KAR may facilitate release of the product from the presumed ELOVL6-KAR complex Homo sapiens