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Literature summary extracted from

  • Villela, A.D.; Ducati, R.G.; Rosado, L.A.; Bloch, C.J.; Prates, M.V.; Goncalves, D.C.; Ramos, C.H.; Basso, L.A.; Santos, D.S.
    Biochemical characterization of uracil phosphoribosyltransferase from Mycobacterium tuberculosis (2013), PLoS ONE, 8, e56445.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.9 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.9 additional information not significantly activated or inhibited by ATP, UTP, and CTP Mycobacterium tuberculosis
2.4.2.9 UMP substrate inhibition at 0.1 mM UMP Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.9 0.0124
-
 Uracil at pH 7.5 and 25°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.9 Mg2+ required Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.9 21289
-
 4 * 21289, calculated from amino acid sequence Mycobacterium tuberculosis
2.4.2.9 22000
-
 4 * 22000, SDS-PAGE Mycobacterium tuberculosis
2.4.2.9 109700
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis the enzyme is specific for uracil UMP + diphosphate
-
 r
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis H37Rv the enzyme is specific for uracil UMP + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Mycobacterium tuberculosis
-
-
-
2.4.2.9 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.9 DEAE Sepharose column chromatography, Mono Q column chromatography, and Sephacryl S-300 gel filtration Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.9 0.76
-
 crude extract, at pH 7.5 and 25°C Mycobacterium tuberculosis
2.4.2.9 1.56
-
 after 2.1fold purification, at pH 7.5 and 25°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate the enzyme is specific for uracil Mycobacterium tuberculosis UMP + diphosphate
-
 r
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate the enzyme is specific for uracil Mycobacterium tuberculosis H37Rv UMP + diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.9 homotetramer 4 * 22000, SDS-PAGE Mycobacterium tuberculosis
2.4.2.9 homotetramer 4 * 21289, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.4.2.9 UPRT
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.9 0.65
-
 Uracil at pH 7.5 and 25°C Mycobacterium tuberculosis