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Literature summary extracted from

  • Dziadek, B.; Brzostek, A.; Grzybowski, M.; Fol, M.; Krupa, A.; Kryczka, J.; Plocinski, P.; Kurdowska, A.; Dziadek, J.
    Mycobacterium tuberculosis AtsG (Rv0296c), GlmU (Rv1018c) and SahH (Rv3248c) proteins function as the human IL-8-binding effectors and contribute to pathogen entry into human neutrophils (2016), PLoS ONE, 11, e0148030.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.157 medicine arylsulphatase AtsG , bifunctional glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyl transferase GlmU and S-adenosyl-L-homocysteine hydrolase SahH are the Mycobacterium tuberculosis proteins that bind to human IL-8. The interactions with IL-8 are characterized by high binding affinity with KD values of 6.83x10-6 M, 5.24x10-6 M and 7.14x10-10 M, respectively. Strains overproducing the enzymes show a significantly increased number of intracellularly located bacilli compared with those of wild-type Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.157 gene glmU, recombinant expression of His-tagged enzyme in Escherichia coli strain BL12(DE3) Mycobacterium tuberculosis
2.7.7.23 gene glmU, recombinant expression of His-tagged enzyme in Escherichia coli strain BL12(DE3), overexpression of GlmU in Mycobacterium tuberculosis Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.157 additional information construction of Mycobacterium tuberculosis mutant strains overproducing GlmU allows determination of the contribution of the protein to mycobacterial entry into human neutrophils Mycobacterium tuberculosis
2.7.7.23 additional information construction of Mycobacterium tuberculosis mutant strains overproducing GlmU allows determination of the contribution of the protein to mycobacterial entry into human neutrophils Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Mycobacterium tuberculosis
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Mycobacterium tuberculosis H37Rv
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Mycobacterium tuberculosis ATCC 25618
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Mycobacterium tuberculosis
-
diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Mycobacterium tuberculosis H37Rv
-
diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Mycobacterium tuberculosis ATCC 25618
-
diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.157 Mycobacterium tuberculosis P9WMN3
-
-
2.3.1.157 Mycobacterium tuberculosis ATCC 25618 P9WMN3
-
-
2.3.1.157 Mycobacterium tuberculosis H37Rv P9WMN3
-
-
2.7.7.23 Mycobacterium tuberculosis P9WMN3
-
-
2.7.7.23 Mycobacterium tuberculosis H37Rv P9WMN3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.157 recombinant His-tagged enzyme from Escherichia coli strain BL12(DE3) by cobalt affinity chromatography, overexpression of GlmU in Mycobacterium tuberculosis Mycobacterium tuberculosis
2.7.7.23 recombinant His-tagged enzyme from Escherichia coli strain BL12(DE3) by cobalt affinity chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
Mycobacterium tuberculosis CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
Mycobacterium tuberculosis H37Rv CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
Mycobacterium tuberculosis ATCC 25618 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
?
2.3.1.157 additional information the recombinant enzyme binds to human interleukin-8, interaction analysis with human neutrophils, overview Mycobacterium tuberculosis ?
-
?
2.3.1.157 additional information the recombinant enzyme binds to human interleukin-8, interaction analysis with human neutrophils, overview Mycobacterium tuberculosis H37Rv ?
-
?
2.3.1.157 additional information the recombinant enzyme binds to human interleukin-8, interaction analysis with human neutrophils, overview Mycobacterium tuberculosis ATCC 25618 ?
-
?
2.7.7.23 additional information the recombinant enzyme binds to human interleukin-8, interaction analysis with human neutrophils, overview Mycobacterium tuberculosis ?
-
?
2.7.7.23 additional information the recombinant enzyme binds to human interleukin-8, interaction analysis with human neutrophils, overview Mycobacterium tuberculosis H37Rv ?
-
?
2.7.7.23 additional information the recombinant enzyme binds to human interleukin-8, interaction analysis with human neutrophils, overview Mycobacterium tuberculosis ATCC 25618 ?
-
?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
Mycobacterium tuberculosis diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
Mycobacterium tuberculosis H37Rv diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
Mycobacterium tuberculosis ATCC 25618 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.157 GlmU
-
Mycobacterium tuberculosis
2.3.1.157 glucosamine-1-phosphate acetyltransferase
-
Mycobacterium tuberculosis
2.3.1.157 Rv1018c
-
Mycobacterium tuberculosis
2.7.7.23 GlmU
-
Mycobacterium tuberculosis
2.7.7.23 N-acetylglucosamine-1-phosphate uridyltransferase
-
Mycobacterium tuberculosis
2.7.7.23 Rv1018c
-
Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.157 acetyl-CoA
-
Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.3.1.157 physiological function the enzyme is one of the pathogen proteins that bind to human interleukin-8, IL-8. The binding interaction of mycobacterial proteins AtsG, GlmU and SahH with human IL-8 may indicate that these proteins participate in the modulation of the early events of infection with tubercle bacilli and could affect pathogen attachment to target cells. Interleukin-8 belongs to the family of CXC chemokines and functions as a chemoattractant and activator of different subsets of leukocytes Mycobacterium tuberculosis
2.7.7.23 physiological function the enzyme is one of the pathogen proteins that bind to human interleukin-8, IL-8. The binding interaction of mycobacterial proteins AtsG, GlmU and SahH with human IL-8 may indicate that these proteins participate in the modulation of the early events of infection with tubercle bacilli and could affect pathogen attachment to target cells. Interleukin-8 belongs to the family of CXC chemokines and functions as a chemoattractant and activator of different subsets of leukocytes Mycobacterium tuberculosis