Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kikuta, A.; Furukawa, E.; Ogawa, R.; Suganuma, N.; Saitoh, M.; Nishimaki, T.; Katsumura, T.; Oota, H.; Kawamoto, T.; Tatsukawa, H.; Hashimoto, H.; Hitomi, K.
    biochemical characterization of medaka (Oryzias latipes) transglutaminases, OlTGK1 and OlTGK2, as orthologues of human keratinocyte-type transglutaminase (2015), PLoS ONE, 10, e0144194.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.13 expressed in Escherichia coli BL21(DE3)LysS cells Oryzias latipes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ required Oryzias latipes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 80000
-
 x * 80000, isoform TGK1, SDS-PAGE Oryzias latipes
2.3.2.13 100000
-
 x * 100000, isoform TGK2, SDS-PAGE Oryzias latipes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.13 [protein]-L-glutamine + alkylamine Oryzias latipes
-
 [protein]-N5-alkyl-L-glutamine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Oryzias latipes A0A0S3Q197
-
-
2.3.2.13 Oryzias latipes A0A0S3Q1B1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 Talon column chromatography and Superdex 200 gel filtration Oryzias latipes

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.13 epidermis
-
 Oryzias latipes
-
2.3.2.13 intervertebral disk
-
 Oryzias latipes
-
2.3.2.13 pancreas isoform TGK1 Oryzias latipes
-
2.3.2.13 pharyngeal tooth
-
 Oryzias latipes
-
2.3.2.13 skin
-
 Oryzias latipes
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 [casein]-L-glutamine + biotin-labeled pentylamine
-
 Oryzias latipes ? + NH3
-
 ?
2.3.2.13 [protein]-L-glutamine + alkylamine
-
 Oryzias latipes [protein]-N5-alkyl-L-glutamine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 ? x * 100000, isoform TGK2, SDS-PAGE Oryzias latipes
2.3.2.13 ? x * 80000, isoform TGK1, SDS-PAGE Oryzias latipes

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 TGK1 isoform Oryzias latipes
2.3.2.13 TGK2 isoform Oryzias latipes
2.3.2.13 transglutaminase
-
 Oryzias latipes