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Literature summary extracted from

  • Liu, Y.; Dun, B.; Shi, P.; Ma, R.; Luo, H.; Bai, Y.; Xie, X.; Yao, B.
    A novel GH7 endo-beta-1,4-glucanase from Neosartorya fischeri P1 with good thermostability, broad substrate specificity and potential application in the brewing industry (2015), PLoS ONE, 10, e0137485.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.4 degradation under simulated mashing conditions, addition of Cel7A (99 microg) reduces the mash viscosity by 9.1% and filtration time by 24.6% Aspergillus fischeri

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Pichia pastoris Aspergillus fischeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Ag+ 5 mM, no residual activity Aspergillus fischeri
3.2.1.4 Co2+ 5 mM, 90% of initial activity Aspergillus fischeri
3.2.1.4 EDTA 5 mM, 76% of initial activity Aspergillus fischeri
3.2.1.4 Mn2+ 5 mM, 44% of initial activity Aspergillus fischeri
3.2.1.4 Na+ 5 mM, 65% of initial activity Aspergillus fischeri
3.2.1.4 Pb2+ 5 mM, 56% of initial activity Aspergillus fischeri
3.2.1.4 sodium dodecylsulfate 5 mM, 5% of initial activity Aspergillus fischeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ca2+ 5 mM, 119% of initial activity Aspergillus fischeri
3.2.1.4 Fe3+ 5 mM, 132% of initial activity Aspergillus fischeri

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Aspergillus fischeri A0A0N7E7X2 i.e. Aspergillus fischeri
-
3.2.1.4 Aspergillus fischeri P1 A0A0N7E7X2 i.e. Aspergillus fischeri
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 proteolytic modification sequence contains a putative signal peptide at residues 1-20 Aspergillus fischeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 2020
-
 substrate barley beta-glucan, pH 5.0, 60°C Aspergillus fischeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 barley beta-glucan + H2O
-
 Aspergillus fischeri ?
-
 ?
3.2.1.4 barley beta-glucan + H2O
-
 Aspergillus fischeri P1 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O 18.6% of the activity with barley beta-glucan Aspergillus fischeri ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O 18.6% of the activity with barley beta-glucan Aspergillus fischeri P1 ?
-
 ?
3.2.1.4 laminarin + H2O 9.1% of the activity with barley beta-glucan Aspergillus fischeri ?
-
 ?
3.2.1.4 laminarin + H2O 9.1% of the activity with barley beta-glucan Aspergillus fischeri P1 ?
-
 ?
3.2.1.4 lichenan + H2O 53.2% of the activity with barley beta-glucan Aspergillus fischeri ?
-
 ?
3.2.1.4 lichenan + H2O 53.2% of the activity with barley beta-glucan Aspergillus fischeri P1 ?
-
 ?
3.2.1.4 additional information enzyme displays weak activity on weak on locust bean galactomannan, avicel, and filter paper Aspergillus fischeri ?
-
 ?
3.2.1.4 additional information enzyme displays weak activity on weak on locust bean galactomannan, avicel, and filter paper Aspergillus fischeri P1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Cel7A
-
 Aspergillus fischeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60
-
-
 Aspergillus fischeri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 60
-
 stable Aspergillus fischeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5
-
-
 Aspergillus fischeri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 3 6
-
 Aspergillus fischeri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 3 8 stable Aspergillus fischeri