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Literature summary extracted from

  • Kajala, I.; Shi, Q.; Nyyssoelae, A.; Maina, N.H.; Hou, Y.; Katina, K.; Tenkanen, M.; Juvonen, R.
    Cloning and characterization of a Weissella confusa dextransucrase and its application in high fibre baking (2015), PLoS ONE, 10, e0116418.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.5 food industry the enzyme from Weissella confusa strain VTT E-90392 is useful in dextran production during sour dough production. The hydrocolloidal properties of dextran can facilitate a more substantial use of wheat bran and counter the negative effects of bran on bread quality, optimization of enzymatic dextran production in wheat bran Weissella confusa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.5 gene dsr, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Lactococcus lactis Weissella confusa

General Stability

EC Number General Stability Organism
2.4.1.5 glycerol enhances the stability at 25-40°C, enzyme stability at 35°C with glycerol is higher than at 25°C without glycerol Weissella confusa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.5 additional information
-
 additional information Michaelis-Menten kinetics Weissella confusa
2.4.1.5 17.4
-
 sucrose pH 5.4, 35°C, recombinant enzyme Weissella confusa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.5 Ca2+ required Weissella confusa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.5 160000
-
 x * 160000, recombinant His-tagged enzyme, SDS-PAGE Weissella confusa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n Weissella confusa substrate is dextran D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n Weissella confusa VTT E-90392 substrate is dextran D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Weissella confusa X4QKX0
-
-
2.4.1.5 Weissella confusa VTT E-90392 X4QKX0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 recombinant His-tagged enzyme from Lactococcus lactis by nickel affinity chromatography Weissella confusa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.5 7.2
-
 recombinant His-tagged enzme, by the 14C-sucrose radioisotope assay, pH 5.4, 35°C Weissella confusa
2.4.1.5 18.9
-
 recombinant His-tagged enzme, by and Nelson-Somogyi assay, pH 5.4, 35°C Weissella confusa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 additional information optimization of enzymatic dextran production in wheat bran, overview Weissella confusa ?
-
 ?
2.4.1.5 additional information optimization of enzymatic dextran production in wheat bran, overview Weissella confusa VTT E-90392 ?
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n substrate is dextran Weissella confusa D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n substrate is dextran Weissella confusa D-fructose + [(1->6)-alpha-D-glucosyl]n+1 product determination by NMR spectroscopy ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n substrate is dextran Weissella confusa VTT E-90392 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n substrate is dextran Weissella confusa VTT E-90392 D-fructose + [(1->6)-alpha-D-glucosyl]n+1 product determination by NMR spectroscopy ?

Subunits

EC Number Subunits Comment Organism
2.4.1.5 ? x * 160000, recombinant His-tagged enzyme, SDS-PAGE Weissella confusa
2.4.1.5 More enzyme tertiary structure modeling Weissella confusa

Synonyms

EC Number Synonyms Comment Organism
2.4.1.5 Wc392-rDSR
-
 Weissella confusa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 35
-
 recombinant enzyme Weissella confusa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.5 25 45 over 65% activity within this range, purified recombinant enzyme Weissella confusa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.5 35 40 enzyme stability decreases sharply when the temperature is raised from 35°C to 40°C, half-life at 35°C is 26.3 h and at 40°C 7.4 h without glycerol, and 37.5 h and 11.2 h with glycerol, respectively Weissella confusa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.5 23.2
-
 sucrose pH 5.4, 35°C, recombinant enzyme Weissella confusa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.4
-
 recombinant enzyme Weissella confusa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.5 4 6.5 over 60% activity within this range, purified recombinant enzyme Weissella confusa

General Information

EC Number General Information Comment Organism
2.4.1.5 evolution sequence comparison with sequence alignment of Weissella confusa LBAE C39-2 dextransucrase and Lactobacillus reuteri 180, GTF180 Weissella confusa
2.4.1.5 additional information structure and size of in vitro dextran product is similar to dextran produced in vivo. Enzymatic production of dextran into wheat bran, overview Weissella confusa