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Literature summary extracted from

  • Rinaldo, A.R.; Cavallini, E.; Jia, Y.; Moss, S.M.; McDavid, D.A.; Hooper, L.C.; Robinson, S.P.; Tornielli, G.B.; Zenoni, S.; Ford, C.M.; Boss, P.K.; Walker, A.R.
    A grapevine anthocyanin acyltransferase, transcriptionally regulated by VvMYBA, can produce most acylated anthocyanins present in grape skins (2015), Plant Physiol., 169, 1897-1916.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.215 expression in Escherichia coli Vitis vinifera

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.215 additional information protein sequence changes and gene expression patterns of 3AT correlate with the absence of acylated anthocyanins in cv Pinot Noir and pale-fruited cv Cabernet Sauvignon mutants Vitis vinifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.215 0.0012
-
 coumaroyl-CoA pH 6.5, 30°C Vitis vinifera
2.3.1.215 0.0041
-
 caffeoyl-CoA pH 6.5, 30°C Vitis vinifera
2.3.1.215 0.0169
-
 acetyl-CoA pH 6.5, 30°C Vitis vinifera
2.3.1.215 0.064
-
 cyanidin 3-O,5-O-beta-D-diglucoside pH 6.5, 30°C Vitis vinifera
2.3.1.215 0.079
-
 peonidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
2.3.1.215 0.098
-
 malvidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
2.3.1.215 0.506
-
 cyanidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
2.3.1.215 0.588
-
 malonyl-CoA pH 6.5, 30°C Vitis vinifera
2.3.1.215 0.765
-
 delphinidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.215 Vitis vinifera D7TU67
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.215 cyanidin 3-O,5-O-beta-D-diglucoside + acetyl-CoA
-
 Vitis vinifera CoA + cyanidin-3-O-(6-O-acetyl)-beta-D-glucosyl-5-O-beta-D-glucoside
-
 ?
2.3.1.215 cyanidin 3-O-beta-D-glucoside + acetyl-CoA
-
 Vitis vinifera CoA + cyanidin 3-O-(6-O-acetyl)-beta-D-glucoside
-
 ?
2.3.1.215 delphinidin 3-O-beta-D-glucoside + acetyl-CoA
-
 Vitis vinifera CoA + delphinidin 3-O-(6-O-acetyl)-beta-D-glucoside
-
 ?
2.3.1.215 malvidin 3-O-beta-D-glucoside + acetyl-CoA
-
 Vitis vinifera CoA + malvidin 3-O-(6-O-acetyl)-beta-D-glucoside
-
 ?
2.3.1.215 malvidin 3-O-beta-D-glucoside + caffeoyl-CoA
-
 Vitis vinifera CoA + malvidin 3-O-(6-O-caffeoyl)-beta-D-glucoside
-
 ?
2.3.1.215 malvidin 3-O-beta-D-glucoside + coumaroyl-CoA
-
 Vitis vinifera CoA + malvidin 3-O-(6-O-coumaroyl)-beta-D-glucoside
-
 ?
2.3.1.215 malvidin 3-O-beta-D-glucoside + malonyl-CoA
-
 Vitis vinifera CoA + malvidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
2.3.1.215 additional information no substrate: cyanidin 3-rutinoside Vitis vinifera ?
-
 ?
2.3.1.215 peonidin 3-O-beta-D-glucoside + acetyl-CoA
-
 Vitis vinifera CoA + peonidin 3-O-(6-O-acetyl)-beta-D-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.215 3AT
-
 Vitis vinifera
2.3.1.215 anthocyanidin 3-O-glucoside 6''-O-acyltransferase
-
 Vitis vinifera
2.3.1.215 VIT_03s0017g00870
-
 Vitis vinifera

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.215 0.13
-
 coumaroyl-CoA pH 6.5, 30°C Vitis vinifera
2.3.1.215 0.18
-
 cyanidin 3-O,5-O-beta-D-diglucoside pH 6.5, 30°C Vitis vinifera
2.3.1.215 0.29
-
 caffeoyl-CoA pH 6.5, 30°C Vitis vinifera
2.3.1.215 0.92
-
 malonyl-CoA pH 6.5, 30°C Vitis vinifera
2.3.1.215 1.11
-
 acetyl-CoA pH 6.5, 30°C Vitis vinifera
2.3.1.215 13.92
-
 malvidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
2.3.1.215 14.2
-
 peonidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
2.3.1.215 67.89
-
 delphinidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
2.3.1.215 77
-
 cyanidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera

Expression

EC Number Organism Comment Expression
2.3.1.215 Vitis vinifera transcription factor MYBA is a positive regulator of the later stages of anthocyanin synthesis, modification, and transport in cv Shiraz. Anthocyanidin 3-O-glucoside 6''-O-acyltransferase is upregulated by MYBA additional information

General Information

EC Number General Information Comment Organism
2.3.1.215 physiological function constitutive expression of 3AT in tobacco results in the production of acylated anthocyanins Vitis vinifera

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.215 1.6
-
 malonyl-CoA pH 6.5, 30°C Vitis vinifera
2.3.1.215 3
-
 cyanidin 3-O,5-O-beta-D-diglucoside pH 6.5, 30°C Vitis vinifera
2.3.1.215 70
-
 caffeoyl-CoA pH 6.5, 30°C Vitis vinifera
2.3.1.215 80
-
 acetyl-CoA pH 6.5, 30°C Vitis vinifera
2.3.1.215 90
-
 delphinidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
2.3.1.215 100
-
 coumaroyl-CoA pH 6.5, 30°C Vitis vinifera
2.3.1.215 150
-
 cyanidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
2.3.1.215 150
-
 malvidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera
2.3.1.215 180
-
 peonidin 3-O-beta-D-glucoside pH 6.5, 30°C Vitis vinifera