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Literature summary extracted from

  • Ewald, R.; Hoffmann, C.; Florian, A.; Neuhaus, E.; Fernie, A.R.; Bauwe, H.
    Lipoate-protein ligase and octanoyltransferase are essential for protein lipoylation in mitochondria of Arabidopsis (2014), Plant Physiol., 165, 978-990.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.20 expressed in the lipoylation-deficient Escherichia coli mutant TM137 and in Nicotiana tabacum protoplasts Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.181 mitochondrion
-
 Arabidopsis thaliana 5739
-
6.3.1.20 mitochondrion
-
 Arabidopsis thaliana 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.20 30000
-
 x * 30000, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.20 ATP + (R)-lipoate Arabidopsis thaliana
-
 lipoyl-AMP + diphosphate
-
 ?
6.3.1.20 lipoyl-AMP + [GLDH1 protein]-L-lysine Arabidopsis thaliana
-
 [GLDH1 protein]-N6-(lipoyl)lysine + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.181 Arabidopsis thaliana Q9SXP7
-
-
6.3.1.20 Arabidopsis thaliana
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.20 leaf
-
 Arabidopsis thaliana
-
6.3.1.20 root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.181 additional information pyruvate dehydrogenase E2 domain is exclusively octanoylated by lipoate-protein ligase LPLA, EC 2.7.7.63 Arabidopsis thaliana ?
-
 ?
2.3.1.181 octanoyl-[acyl-carrier protein] + alpha-ketoglutarate dehydrogenase apo-E2 domain
-
 Arabidopsis thaliana alpha-ketoglutarate dehydrogenase E2-domain N6-(octanoyl)lysine + acyl-carrier protein
-
 ?
2.3.1.181 octanoyl-[acyl-carrier protein] + glycine dehydrogenase H-protein
-
 Arabidopsis thaliana glycine dehydrogenase H-protein N6-(octanoyl)lysine + acyl-carrier protein glycine dehydrogenase H-protein can be octanoylated both by Lip2 or by lipoate-protein ligase LPLA, EC 2.7.7.63 ?
6.3.1.20 ATP + (R)-lipoate
-
 Arabidopsis thaliana lipoyl-AMP + diphosphate
-
 ?
6.3.1.20 lipoyl-AMP + [GLDH1 protein]-L-lysine
-
 Arabidopsis thaliana [GLDH1 protein]-N6-(lipoyl)lysine + AMP
-
 ?
6.3.1.20 lipoyl-AMP + [pyruvate dehydrogenase-E2]-L-lysine
-
 Arabidopsis thaliana [pyruvate dehydrogenase-E2]-N6-(lipoyl)lysine + AMP
-
 ?
6.3.1.20 lipoyl-AMP + [TM137 soluble protein]-L-lysine
-
 Arabidopsis thaliana [TM137 soluble protein]-N6-(lipoyl)lysine + AMP
-
 ?
6.3.1.20 additional information the enzyme uses octanoyl-nucleoside monophosphate and possibly other donor substrates for the octanoylation of mitochondrial pyruvate dehydrogenase-E2 (essentially) and glycine decarboxylase H-protein, and shows no reactivity with bacterial and possibly plant alpha- ketoglutarate dehydrogenase-E2 Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.20 ? x * 30000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.3.1.181 LIP2
-
 Arabidopsis thaliana
6.3.1.20 LPLA
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
6.3.1.20 physiological function the enzyme is essential for embryo development and necessary for the lipoylation of pyruvate dehydrogenase-E2 proteins in leaves and roots Arabidopsis thaliana