Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hao, G.; Shi, R.; Tao, R.; Fang, Q.; Jiang, X.; Ji, H.; Feng, L.; Huang, L.
    Cloning, molecular characterization and functional analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) gene for diterpenoid tanshinone biosynthesis in Salvia miltiorrhiza Bge. f. alba (2013), Plant Physiol. Biochem., 70, 21-32.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.7.4 gene HDR, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, functional complementation by SmHDR1 of the Escherichia coli HDR mutant MG1655 ara/ispH, overexpression of SmHDR1 accelerates the biosynthesis of beta-carotene and enhances the production of tanshinones in cultured hairy roots of Salvia miltiorrhiza Bge. f. alba, transformation using Agrobacterium rhizogenes strain ACCC10060 Salvia miltiorrhiza f. alba

Protein Variants

EC Number Protein Variants Comment Organism
1.17.7.4 additional information recombinant overexpression of the enzyme leads to increased tanshinone content in SmHDR1 transgenic hairy roots, overview. The total levels of all three tanshinones are 0.70 to 1.04fold higher in the H lines than in the wild-type Salvia miltiorrhiza f. alba

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.7.4 chloroplast the enzyme has a chloroplast transit peptide, the cleavage site of the putative N-terminal cTP of SmHDR1 is predicted to be located between Arg33 and Cys34, comprising 33 amino acid residues Salvia miltiorrhiza f. alba 9507
-
1.17.7.4 additional information no transmembrane regions in the SmHDR1 polypeptide Salvia miltiorrhiza f. alba
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.7.4 Iron-sulfur cluster the iron-sulfur bridge is involved in catalysis Salvia miltiorrhiza f. alba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.7.4 51860
-
 x * 51860, sequence calculation Salvia miltiorrhiza f. alba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Salvia miltiorrhiza f. alba
-
 isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Salvia miltiorrhiza f. alba
-
 dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.7.4 Salvia miltiorrhiza f. alba T1RJ09 gene HDR
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.7.4 hairy root
-
 Salvia miltiorrhiza f. alba
-
1.17.7.4 leaf highest expression level Salvia miltiorrhiza f. alba
-
1.17.7.4 root
-
 Salvia miltiorrhiza f. alba
-
1.17.7.4 stem
-
 Salvia miltiorrhiza f. alba
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Salvia miltiorrhiza f. alba isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Salvia miltiorrhiza f. alba dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.7.4 ? x * 51860, sequence calculation Salvia miltiorrhiza f. alba

Synonyms

EC Number Synonyms Comment Organism
1.17.7.4 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase
-
 Salvia miltiorrhiza f. alba
1.17.7.4 HDR
-
 Salvia miltiorrhiza f. alba

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.7.4 Ferredoxin
-
 Salvia miltiorrhiza f. alba

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.17.7.4 Salvia miltiorrhiza f. alba sequence calculation
-
 5.81

Expression

EC Number Organism Comment Expression
1.17.7.4 Salvia miltiorrhiza f. alba expression of gene SmHDR1 is induced by 0.1 mM methyl-jasmonate and salicylic acid, but not by 0.1 mM abscisic acid, in hairy roots up

General Information

EC Number General Information Comment Organism
1.17.7.4 physiological function enzyme SmHDR1 is HDR is involved in the plastid nonmevalonate pathway of isoprenoid biosynthesis, it is an important enzyme involved in the biosynthesis of diterpenoid tanshinones in Salvia miltiorrhiza Bge. f. alba, HDR is key enzyme in the biosynthesis of precursors of isoprenoids Salvia miltiorrhiza f. alba