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Literature summary extracted from

  • Vishwakarma, R.; Rub, R.; Singh, S.; Sonawane, P.; Srivastava, S.; Kumari, U.; Santosh Kumar, R.; Khan, B.
    Molecular cloning, biochemical characterization, and differential expression of an acetyl-CoA C-acetyltransferase gene (AACT) of Brahmi (Bacopa monniera) (2013), Plant Mol. Biol. Rep., 31, 547-557.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.9 expressed in Escherichia coli BL21(DE3) cells Bacopa monnieri

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.9 Mg2+ inhibitory at concentrations higher than 5 mM Bacopa monnieri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.9 0.02067
-
 acetoacetyl-CoA at pH 8.9 and 25°C Bacopa monnieri
2.3.1.9 0.0258
-
 acetyl-CoA at pH 8.9 and 25°C Bacopa monnieri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.9 Mg2+ maximal activity at 5 mM Bacopa monnieri
2.3.1.9 additional information not stimulated by Ni2+, Ca2+ and Zn2+ Bacopa monnieri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 41320
-
 x * 41320, calculated from amino acid sequence Bacopa monnieri
2.3.1.9 43000
-
 x * 43000, SDS-PAGE Bacopa monnieri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 2 acetyl-CoA Bacopa monnieri
-
 CoA + acetoacetyl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Bacopa monnieri D9U856
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9 Ni-NTA column chromatography and Superdex 75 gel filtration Bacopa monnieri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.9 leaf
-
 Bacopa monnieri
-
2.3.1.9 petal highest expression in roots and petals Bacopa monnieri
-
2.3.1.9 plant pedicel
-
 Bacopa monnieri
-
2.3.1.9 root highest expression in roots and petals Bacopa monnieri
-
2.3.1.9 sepal
-
 Bacopa monnieri
-
2.3.1.9 stem
-
 Bacopa monnieri
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.9 0.9
-
 crude enzyme, at pH 8.9 and 25°C Bacopa monnieri
2.3.1.9 56
-
 after 62.22fold purification, at pH 8.9 and 25°C Bacopa monnieri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 2 acetyl-CoA
-
 Bacopa monnieri CoA + acetoacetyl-CoA
-
 r
2.3.1.9 CoA + acetoacetyl-CoA
-
 Bacopa monnieri 2 acetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.9 ? x * 43000, SDS-PAGE Bacopa monnieri
2.3.1.9 ? x * 41320, calculated from amino acid sequence Bacopa monnieri

Synonyms

EC Number Synonyms Comment Organism
2.3.1.9 AACT
-
 Bacopa monnieri
2.3.1.9 acetoacetyl-CoA C-acetyltransferase
-
 Bacopa monnieri
2.3.1.9 acetoacetyl-CoA thiolase
-
 Bacopa monnieri
2.3.1.9 thiolase II
-
 Bacopa monnieri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.9 3833
-
 acetoacetyl-CoA at pH 8.9 and 25°C Bacopa monnieri

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.9 Bacopa monnieri calculated from amino acid sequence
-
 6.2