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Literature summary extracted from

  • Funaki, A.; Waki, T.; Noguchi, A.; Kawai, Y.; Yamashita, S.; Takahashi, S.; Nakayama, T.
    Identification of a highly specific isoflavone 7-O-glucosyltransferase in the soybean (Glycine max (L.) Merr.) (2015), Plant Cell Physiol., 56, 1512-1520.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.170 gene GmUGT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis Glycine max
2.4.1.170 gene GmUGT3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis Glycine max
2.4.1.170 gene GmUGT4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis Glycine max
2.4.1.170 gene GmUGT7, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis Glycine max
2.4.1.170 gene GmUGT9, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.170 0.00031
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.00042
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.0103
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.0129
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.017
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
2.4.1.170 0.0189
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
2.4.1.170 0.0203
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.0237
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.0267
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.049
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.0537
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
2.4.1.170 0.0564
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.0869
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.0985
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.1637
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.170 UDP-glucose + daidzein Glycine max
-
 UDP + daidzein 7-O-beta-D-glucoside i.e. daidzin ?
2.4.1.170 UDP-glucose + genistein Glycine max
-
 UDP + genistein 7-O-beta-D-glucoside i.e. genistin ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.170 Glycine max
-
 gene GmUGT3
-
2.4.1.170 Glycine max
-
 gene GmUGT4
-
2.4.1.170 Glycine max
-
 gene GmUGT7
-
2.4.1.170 Glycine max
-
 gene GmUGT9
-
2.4.1.170 Glycine max A6BM07 gene GmUGT1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.170 cotyledon
-
 Glycine max
-
2.4.1.170 hypocotyl
-
 Glycine max
-
2.4.1.170 additional information gene GmUGT3 encodes a UGT that is highly specific for isoflavones Glycine max
-
2.4.1.170 additional information gene GmUGT4 encodes a UGT that is highly specific for isoflavones and is mainly expressed in the lateral roots and seeds, followed by taproots, cotyledon, and hypocotyls Glycine max
-
2.4.1.170 additional information gene GmUGT9 encodes a UGT that is highly specific for isoflavones, the levels of the GmUGT9 transcripts are highest in roots, followed by taproots, cotyledon, and hypocotyls Glycine max
-
2.4.1.170 additional information the levels of the most abundant GmUGT1 transcripts in cotyledons are much higher than those in lateral roots Glycine max
-
2.4.1.170 additional information the levels of the most abundant GmUGT7 transcripts in cotyledons are much higher than those in lateral roots Glycine max
-
2.4.1.170 root lateral Glycine max
-
2.4.1.170 root lateral and taproot Glycine max
-
2.4.1.170 seed
-
 Glycine max
-
2.4.1.170 seedling
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.170 additional information
-
 GmUGT1 activity with other flavonoids, overview Glycine max
2.4.1.170 0.045
-
 substrate genistein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
2.4.1.170 0.049
-
 substrate daidzein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
2.4.1.170 0.158
-
 substrate genistein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
2.4.1.170 0.209
-
 substrate genistein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
2.4.1.170 0.253
-
 substrate daidzein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
2.4.1.170 0.253
-
 substrate genistein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
2.4.1.170 0.457
-
 substrate daidzein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
2.4.1.170 0.465
-
 substrate genistein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
2.4.1.170 0.467
-
 substrate daidzein, , pH 8.5, 30°C, purified recombinant enzyme Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.170 additional information GmUGT1 is active with UDP-galactose and UDP-glucuronic acid as substrates. It displays high specific activities toward isoflavones (genistein and daidzein) to produce their 7-O-beta-D-glucosides, it also shows considerable levels of activities toward other flavonoids such as kaempferol, quercetin, naringenin and apigenin. GmUGT1 is also capable of acting on the flavone luteolin, (S)-equol, a 4',7-isoflavandiol, and (R)-equol Glycine max ?
-
 ?
2.4.1.170 additional information in contrast to GmUGT1, UDP-galactose and UDP-glucuronic acid are essentially inert as substrates for GmUGT3, glucosyl-acceptor substrate specificity, overview. No activity with kaempferol, quercetin, naringenin, apigenin, and luteolin Glycine max ?
-
 ?
2.4.1.170 additional information in contrast to GmUGT1, UDP-galactose and UDP-glucuronic acid are essentially inert as substrates for GmUGT4, glucosyl-acceptor substrate specificity, overview. No activity with kaempferol, quercetin, naringenin, apigenin, and luteolin Glycine max ?
-
 ?
2.4.1.170 UDP-glucose + daidzein
-
 Glycine max UDP + daidzein 7-O-beta-D-glucoside i.e. daidzin ?
2.4.1.170 UDP-glucose + genistein
-
 Glycine max UDP + genistein 7-O-beta-D-glucoside i.e. genistin ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.170 GmUGT1
-
 Glycine max
2.4.1.170 GmUGT3
-
 Glycine max
2.4.1.170 GmUGT4
-
 Glycine max
2.4.1.170 GmUGT7
-
 Glycine max
2.4.1.170 GmUGT9
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.170 30
-
 assay at Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.170 0.1
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.11
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.13
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.39
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 0.97
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 1.22
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 1.41
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 1.72
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 1.75
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 4.34
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
2.4.1.170 5.75
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
2.4.1.170 5.89
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 6.04
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 7.65
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 8.79
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.170 8.5
-
 assay at Glycine max

General Information

EC Number General Information Comment Organism
2.4.1.170 evolution nine different GmUGT cDNAs that are related to GmUGT1 are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview. The enzymes of subgroup A, GmUGT3, GmUGT4, and GmUGT9, all are highly specific for isoflavones. Gene GmUGT4, a representative of subgroup A, encodes a UGT that is highly specific for isoflavones and is mainly expressed in the lateral roots and seeds, while gene GmUGT1, a representative of subgroup B, encodes an enzyme displaying a broad glucosyl-acceptor specificity and is expressed in the aerial parts, i.e. cotyledons, hypocotyls and pods Glycine max
2.4.1.170 evolution nine different GmUGT cDNAs that are related to GmUGT1, UniProt ID A6BM07, are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview Glycine max
2.4.1.170 evolution nine different GmUGT cDNAs that are related to GmUGT1, UniProt ID A6BM07, are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview. The enzymes of subgroup A, GmUGT3, GmUGT4, and GmUGT9, all are highly specific for isoflavones Glycine max
2.4.1.170 evolution nine different GmUGT cDNAs that are related to GmUGT1, UniProt ID A6BM07, are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview. The enzymes of subgroup A, GmUGT3, GmUGT4, and GmUGT9, all are highly specific for isoflavones. Gene GmUGT4, a representative of subgroup A, encodes a UGT that is highly specific for isoflavones and is mainly expressed in the lateral roots and seeds, while gene GmUGT1, a representative of subgroup B, encodes an enzyme displaying a broad glucosyl-acceptor specificity and is expressed in the aerial parts, i.e. cotyledons, hypocotyls and pods Glycine max
2.4.1.170 metabolism the isoflavone 7-O-glucosyltransferase is involved in isoflavone conjugate biosynthesis Glycine max
2.4.1.170 physiological function the isoflavone 7-O-glucosyltransferase is involved in isoflavone conjugate biosynthesis. Isoflavone conjugates, 7-O-beta-D-glucosides and 7-O-(6''-malonyl-beta-D-glucosides) of daidzein and genistein, accumulate in soybean roots and serve as the stored precursors of isoflavones (aglycons), which play very important roles in the rhizobia-mediated nodulation of this plant Glycine max
2.4.1.170 physiological function the isoflavone 7-O-glucosyltransferase is involved in isoflavone conjugate biosynthesis. Isoflavone conjugates, 7-O-beta-D-glucosides and 7-O-(6''-malonyl-beta-D-glucosides) of daidzein and genistein, accumulate in soybean roots and serve as the stored precursors of isoflavones (aglycons), which play very important roles in the rhizobia-mediated nodulation of this plant. Isozyme GmUGT4, but not GmUGT1, plays an exclusive role in the conjugation of isoflavones in the lateral roots of the soybean plant Glycine max

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.170 0.25
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 2
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 10.5
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 88
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 94.8
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 124
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 136
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 164
-
 UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
2.4.1.170 167
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 226
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 244
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 255
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
2.4.1.170 291
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2.4.1.170 304
-
 daidzein pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
2.4.1.170 409
-
 genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max