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Literature summary extracted from

  • Wang, H.; Sui, X.; Guo, J.; Wang, Z.; Cheng, J.; Ma, S.; Li, X.; Zhang, Z.
    Antisense suppression of cucumber (Cucumis sativus L.) sucrose synthase 3 (CsSUS3) reduces hypoxic stress tolerance (2014), Plant Cell Environ., 37, 795-810.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.13 additional information when suffering hypoxia stress from flooding, CsSUS3 expression and SUS activity in roots increase, especially in the lateral roots. The soluble SUS activity increases, but the membrane fraction hardly changes Cucumis sativus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.13 gene SUS3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree of plant SUS enzymes, real-time quantitative PCR enzyme expression analysis, antisense expression of SUS3 in Cucumber sativus plants transformed via Agrobacterium tumefaciens strain LBA4404 Cucumis sativus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.13 additional information antisense suppression of Cucumis sativus sucrose synthase 3 reduces hypoxic stress tolerance. Compared with the wild-type cucumbers, the transgenic lines with antisense expression of CsSUS3 are more sensitive to flooding. After 6 days of flooding, the enzyme activity, soluble sugar and UDP-glucose content and the ratio of ATP/ADP in the roots of transgenic plants are significantly lower than that in wild-type plants. The transgenic lines grow more slowly with more yellow necrosis in the leaves, phenotype overview Cucumis sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.13 cytosol
-
 Cucumis sativus 5829
-
2.4.1.13 plasma membrane
-
 Cucumis sativus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.13 NaCl the transcript level and protein abundance of CsSUS3 hardly change during salinity treatment, but the enzyme activity increased slightly, especially on the first and ninth days, compared with control Cucumis sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.13 89000
-
 x * 89000, about, sequence calculation Cucumis sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.13 NDP-glucose + D-fructose Cucumis sativus
-
 NDP + sucrose
-
 r
2.4.1.13 NDP-glucose + D-fructose Cucumis sativus Guonong No. 25
-
 NDP + sucrose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.13 Cucumis sativus K9K7W3
-
-
2.4.1.13 Cucumis sativus Guonong No. 25 K9K7W3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.13 native enzyme from roots partially by dialysis and ultracentrifugation Cucumis sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.13 companion cell
-
 Cucumis sativus
-
2.4.1.13 flower female and male Cucumis sativus
-
2.4.1.13 fruit very low level Cucumis sativus
-
2.4.1.13 phloem
-
 Cucumis sativus
-
2.4.1.13 root enzyme localization in the cells of phloem region in vascular tissues Cucumis sativus
-
2.4.1.13 stem
-
 Cucumis sativus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.13 NDP-glucose + D-fructose
-
 Cucumis sativus NDP + sucrose
-
 r
2.4.1.13 NDP-glucose + D-fructose
-
 Cucumis sativus Guonong No. 25 NDP + sucrose
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.13 ? x * 89000, about, sequence calculation Cucumis sativus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.13 CsSUS3
-
 Cucumis sativus
2.4.1.13 sucrose synthase 3
-
 Cucumis sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.13 7.5
-
 assay at Cucumis sativus

Expression

EC Number Organism Comment Expression
2.4.1.13 Cucumis sativus the transcript level and protein abundance of CsSUS3 hardly change during salinity treatment, but the enzyme activity increased slightly, especially on the first and ninth days, compared with control additional information
2.4.1.13 Cucumis sativus when suffering hypoxia stress from flooding, CsSUS3 expression and SUS activity in roots increase, especially in the lateral roots. The soluble SUS activity increases, but the membrane fraction hardly changes. Drought stress modulated by PEG-6000 causes a transient and slight increase in CsSUS3 transcript level on the first and third days, but decreases similarly to control on the sixth and ninth days up

General Information

EC Number General Information Comment Organism
2.4.1.13 malfunction antisense suppression of Cucumis sativus sucrose synthase 3 reduces hypoxic stress tolerance Cucumis sativus
2.4.1.13 physiological function CsSUS3 participates in resisting hypoxic stress, mechanism analysis Cucumis sativus