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Literature summary extracted from

  • Hettwer, K.; Boettcher, C.; Frolov, A.; Mittasch, J.; Albert, A.; von Roepenack-Lahaye, E.; Strack, D.; Milkowski, C.
    Dynamic metabolic changes in seeds and seedlings of Brassica napus (oilseed rape) suppressing UGT84A9 reveal plasticity and molecular regulation of the phenylpropanoid pathway (2016), Phytochemistry, 124, 46-57.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.120 gene UGT84A9, semi-quantitative RT-PCR, enzyme expression analysis Brassica napus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.120 additional information construction of UGT84A9-suppressing (UGT84A9i) lines, suppression of UGT84A9 under control of the seed-specific NAPINC promoter is maintained in cotyledons during the first two weeks of seedling development. Compared to the wild-type, the UGT84A9i lines display a dramatically altered metabolic phenotype during late seed and early seedling development, in both UGT84A9i lines, the level of sinapine is reduced to about 50% of the wild type level, overview. Determination of metabolite levels in four developmental stages of wild-type and mutants subjected to hierarchical cluster analysis reveal seven metabolite groups Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.120 Brassica napus Q9FYU7 gene UGT84A9
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.120 seed
-
 Brassica napus
-
2.4.1.120 seedling
-
 Brassica napus
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.120 UDP-glucose:sinapic acid glucosyltransferase
-
 Brassica napus
2.4.1.120 UGT84A9
-
 Brassica napus

General Information

EC Number General Information Comment Organism
2.4.1.120 malfunction suppression of the key biosynthetic enzyme UDP-glucose:sinapic acid glucosyltransferase (UGT84A9) inhibits the biosynthesis of sinapine (sinapoylcholine), the major phenolic component of seeds. Suppression of the major sink pathway of sinapic acid impacts the metabolome of developing seeds and seedlings in UGT84A9-suppressing (UGT84A9i) lines, massive alterations become evident in late stages of seed development affecting the accumulation levels of 58 secondary and 7 primary metabolites, e.g. decreased amounts of various hydroxycinnamic acid esters, and increased formation of sinapic and syringic acid glycosides, overview. Suppression of UGT84A9 under control of the seed-specific NAPINC promoter is maintained in cotyledons during the first two weeks of seedling development and associated with a reduced and delayed transformation of sinapine into sinapoylmalate Brassica napus
2.4.1.120 physiological function the enzyme is involved in glycosylation and beta-oxidation as metabolic detoxification strategies to bypass intracellular accumulation of sinapic acid. Feedback regulation of hydroxycinnamic acid biosynthesis Brassica napus