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Literature summary extracted from

  • Paya-Milans, M.; Venegas-Caleron, M.; Salas, J.J.; Garces, R.; Martinez-Force, E.
    Cloning, heterologous expression and biochemical characterization of plastidial sn-glycerol-3-phosphate acyltransferase from Helianthus annuus (2015), Phytochemistry, 111, 27-36.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15 expressed in Escherichia coli XL1-Blue cells Helianthus annuus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.00196
-
 palmitoyl-[acyl-carrier protein] at pH 7.4 and 25°C Helianthus annuus
2.3.1.15 0.00612
-
 stearoyl-[acyl-carrier protein] at pH 7.4 and 25°C Helianthus annuus
2.3.1.15 0.0078
-
 oleoyl-[acyl-carrier protein] at pH 7.4 and 25°C Helianthus annuus
2.3.1.15 0.659
-
 sn-glycerol 3-phosphate at pH 7.4 and 25°C Helianthus annuus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 chloroplast
-
 Helianthus annuus 9507
-
2.3.1.15 plastid
-
 Helianthus annuus 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.15 50000
-
 x * 50000, His-tagged enzyme, SDS-PAGE Helianthus annuus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Helianthus annuus K9JTB0 line CAS-6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 His SpinTrap column chromatography Helianthus annuus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 cotyledon
-
 Helianthus annuus
-
2.3.1.15 leaf
-
 Helianthus annuus
-
2.3.1.15 stem
-
 Helianthus annuus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 oleoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate most active acyl donor Helianthus annuus acyl-carrier protein + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
 Helianthus annuus acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 stearoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate
-
 Helianthus annuus acyl-carrier protein + 1-stearoyl-sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.15 ? x * 50000, His-tagged enzyme, SDS-PAGE Helianthus annuus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 acyl-[acyl carrier protein]:sn-1-glycerol-3-phosphate acyltransferase
-
 Helianthus annuus
2.3.1.15 GPAT
-
 Helianthus annuus
2.3.1.15 PlsB
-
 Helianthus annuus
2.3.1.15 sn-glycerol-3-phosphate acyltransferase
-
 Helianthus annuus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.15 0.00674
-
 stearoyl-[acyl-carrier protein] at pH 7.4 and 25°C Helianthus annuus
2.3.1.15 0.00944
-
 palmitoyl-[acyl-carrier protein] at pH 7.4 and 25°C Helianthus annuus
2.3.1.15 0.011
-
 sn-glycerol 3-phosphate at pH 7.4 and 25°C Helianthus annuus
2.3.1.15 0.0918
-
 oleoyl-[acyl-carrier protein] at pH 7.4 and 25°C Helianthus annuus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 6.9
-
-
 Helianthus annuus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.15 0.017
-
 sn-glycerol 3-phosphate at pH 7.4 and 25°C Helianthus annuus
2.3.1.15 1.1
-
 stearoyl-[acyl-carrier protein] at pH 7.4 and 25°C Helianthus annuus
2.3.1.15 4.82
-
 palmitoyl-[acyl-carrier protein] at pH 7.4 and 25°C Helianthus annuus
2.3.1.15 11.8
-
 oleoyl-[acyl-carrier protein] at pH 7.4 and 25°C Helianthus annuus