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Literature summary extracted from

  • Vande Voorde, J.; Liekens, S.; Gago, F.; Balzarini, J.
    The pyrimidine nucleoside phosphorylase of Mycoplasma hyorhinis and how it may affect nucleoside-based therapy (2014), Nucleosides Nucleotides Nucleic Acids, 33, 394-402.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.2 expressed in Escherichia coli BL21(DE3) pLysS cells Mycoplasma hyorhinis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.2 0.095
-
 uridine at pH 7.6 and 37°C Mycoplasma hyorhinis
2.4.2.2 0.365
-
 thymidine at pH 7.6 and 37°C Mycoplasma hyorhinis
2.4.2.2 0.856
-
 5-trifluorothymidine at pH 7.6 and 37°C Mycoplasma hyorhinis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.2 additional information Mycoplasma hyorhinis the enzyme also displays (2'-deoxy)ribosyltransferase activity ?
-
 ?
2.4.2.2 additional information Mycoplasma hyorhinis ATCC 17981 the enzyme also displays (2'-deoxy)ribosyltransferase activity ?
-
 ?
2.4.2.2 thymidine + phosphate Mycoplasma hyorhinis
-
 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 thymidine + phosphate Mycoplasma hyorhinis ATCC 17981
-
 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate Mycoplasma hyorhinis
-
 uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate Mycoplasma hyorhinis ATCC 17981
-
 uracil + alpha-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.2 Mycoplasma hyorhinis
-
-
-
2.4.2.2 Mycoplasma hyorhinis ATCC 17981
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.2 5-trifluorothymidine + phosphate
-
 Mycoplasma hyorhinis 5-trifluorothymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 5-trifluorothymidine + phosphate
-
 Mycoplasma hyorhinis ATCC 17981 5-trifluorothymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 additional information the enzyme also displays (2'-deoxy)ribosyltransferase activity Mycoplasma hyorhinis ?
-
 ?
2.4.2.2 additional information the enzyme also displays (2'-deoxy)ribosyltransferase activity Mycoplasma hyorhinis ATCC 17981 ?
-
 ?
2.4.2.2 thymidine + phosphate
-
 Mycoplasma hyorhinis thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 thymidine + phosphate
-
 Mycoplasma hyorhinis ATCC 17981 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Mycoplasma hyorhinis uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Mycoplasma hyorhinis ATCC 17981 uracil + alpha-D-ribose 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.2 PYNP
-
 Mycoplasma hyorhinis
2.4.2.2 pyrimidine nucleoside phosphorylase
-
 Mycoplasma hyorhinis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.2 8.25
-
 5-trifluorothymidine at pH 7.6 and 37°C Mycoplasma hyorhinis
2.4.2.2 9.03
-
 uridine at pH 7.6 and 37°C Mycoplasma hyorhinis
2.4.2.2 20.5
-
 thymidine at pH 7.6 and 37°C Mycoplasma hyorhinis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.2 10
-
 5-trifluorothymidine at pH 7.6 and 37°C Mycoplasma hyorhinis
2.4.2.2 56.7
-
 thymidine at pH 7.6 and 37°C Mycoplasma hyorhinis
2.4.2.2 95
-
 uridine at pH 7.6 and 37°C Mycoplasma hyorhinis