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Literature summary extracted from

  • Mueller, M.; Hartmann, M.; Schuster, I.; Bender, S.; Thuering, K.L.; Helm, M.; Katze, J.R.; Nellen, W.; Lyko, F.; Ehrenhofer-Murray, A.E.
    Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine (2015), Nucleic Acids Res., 43, 10952-10962.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.203 queuine PMT1 is strongly stimulated by prior queuosine (Q) modification of the substrate tRNA. In vivo tRNA methylation levels are stimulated by growth of cells in queuine-containing medium, in vitro Pmt1 activity is enhanced on Q-containing RNA; and queuine-stimulated in vivo methylation is abrogated by the absence of the eukaryotic tRNA-guanine transglycosylase Schizosaccharomyces pombe
2.1.1.203 queuine the addition of queuine to the growth medium increases C38 methylation from 10 to 100%, while other cytosines remain unaffected Dictyostelium discoideum
2.1.1.204 queuine a micronutrient that is scavenged by higher eukaryotes from the diet and gut microflora. The enzyme is strongly stimulated by prior queuosine modification of the substrate tRNA. In vivo tRNA methylation levels are stimulated by growth of cells in queuine-containing medium Schizosaccharomyces pombe
2.1.1.204 queuine a micronutrient that is scavenged by higher eukaryotes from the diet and gut microflora. The enzyme is strongly stimulated by prior queuosine modification of the substrate tRNA. In vivo tRNA methylation levels are stimulated by growth of cells in queuine-containing medium Dictyostelium discoideum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.204 overexpression of GFP-tagged DnmA in Dictyostelium discoideum Dictyostelium discoideum

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.204 additional information generation of a Prmt1-deletion mutant Schizosaccharomyces pombe
2.1.1.204 additional information generation of a DnmA-deletion mutant Dictyostelium discoideum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.204 Mg2+ required Dictyostelium discoideum
2.1.1.204 Mg2+ required Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.204 additional information Schizosaccharomyces pombe Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs. Pmt1 shows a striking selectivity for tRNAAsp methylation, which distinguishes Pmt1 from other Dnmt2 homologues. C34 of tRNAPro is a Pmt1- and queuosine-independent tRNA methylation site in Schizosaccharomyces pombe ?
-
 ?
2.1.1.204 additional information Dictyostelium discoideum Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs ?
-
 ?
2.1.1.204 S-adenosyl-L-methionine + cytosine38 in tRNA Dictyostelium discoideum
-
 S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
 ?
2.1.1.204 S-adenosyl-L-methionine + cytosine38 in tRNAAsp Schizosaccharomyces pombe
-
 S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.203 Dictyostelium discoideum Q54JH6 cf. EC 2.1.1.37
-
2.1.1.203 Schizosaccharomyces pombe P40999 cf. EC 2.1.1.204
-
2.1.1.203 Schizosaccharomyces pombe 972 P40999 cf. EC 2.1.1.204
-
2.1.1.204 Dictyostelium discoideum
-
-
-
2.1.1.204 Schizosaccharomyces pombe
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.203 S-adenosyl-L-methionine + cytosine34 in tRNAPro
-
 Schizosaccharomyces pombe S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNAPro
-
 ?
2.1.1.203 S-adenosyl-L-methionine + cytosine34 in tRNAPro
-
 Dictyostelium discoideum S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNAPro
-
 ?
2.1.1.203 S-adenosyl-L-methionine + cytosine34 in tRNAPro
-
 Schizosaccharomyces pombe 972 S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNAPro
-
 ?
2.1.1.204 additional information Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs. Pmt1 shows a striking selectivity for tRNAAsp methylation, which distinguishes Pmt1 from other Dnmt2 homologues. C34 of tRNAPro is a Pmt1- and queuosine-independent tRNA methylation site in Schizosaccharomyces pombe Schizosaccharomyces pombe ?
-
 ?
2.1.1.204 additional information Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs Dictyostelium discoideum ?
-
 ?
2.1.1.204 additional information in vitro methylation activity on tRNAAsp and, to a lesser extent, tRNAGlu. No methylation activity with C38-containing tRNAs for histidine, glutamate, and valine Schizosaccharomyces pombe ?
-
 ?
2.1.1.204 S-adenosyl-L-methionine + cytosine38 in tRNA
-
 Dictyostelium discoideum S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
 ?
2.1.1.204 S-adenosyl-L-methionine + cytosine38 in tRNAAsp
-
 Schizosaccharomyces pombe S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
 ?
2.1.1.204 S-adenosyl-L-methionine + cytosine38 in tRNAAsp
-
 Dictyostelium discoideum S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
 ?
2.1.1.204 S-adenosyl-L-methionine + cytosine38 in tRNAGlu 25% of the activity with tRNAAsp, the activity with tRNAGlu is not affected by the presence of peptone Schizosaccharomyces pombe S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlu
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.203 DnmA
-
 Dictyostelium discoideum
2.1.1.203 PMT1
-
 Schizosaccharomyces pombe
2.1.1.203 PMT1
-
 Dictyostelium discoideum
2.1.1.204 DnmA
-
 Dictyostelium discoideum
2.1.1.204 Dnmt2
-
 Schizosaccharomyces pombe
2.1.1.204 Dnmt2
-
 Dictyostelium discoideum
2.1.1.204 PMT1
-
 Schizosaccharomyces pombe
2.1.1.204 pombe methyltransferase 1
-
 Schizosaccharomyces pombe

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.204 22
-
 assay at Dictyostelium discoideum
2.1.1.204 22
-
 assay at Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.204 7.5
-
 assay at Schizosaccharomyces pombe
2.1.1.204 7.5
-
 assay at Dictyostelium discoideum

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.204 S-adenosyl-L-methionine
-
 Schizosaccharomyces pombe
2.1.1.204 S-adenosyl-L-methionine
-
 Dictyostelium discoideum

Expression

EC Number Organism Comment Expression
2.1.1.204 Schizosaccharomyces pombe cultivation of Schizosaccharomyces pombe in minimal medium leads to approximately 23% of tRNAAsp methylation down
2.1.1.204 Schizosaccharomyces pombe methylation is not enhanced by stress conditions such as glucose starvation, heat stress or oxidative stress additional information
2.1.1.204 Schizosaccharomyces pombe tRNAAsp methylation is induced to 100% when cells are grown in the presence of peptone up

General Information

EC Number General Information Comment Organism
2.1.1.204 malfunction methylation at C11 of tRNASer (GCT) shows an inverse correlation to the presence of Pmt1 and queuine in pmt1-mutant cells in that its level increases from 23 to 37% in the absence of Pmt1 Schizosaccharomyces pombe
2.1.1.204 physiological function Pmt1 provides in vivo tRNA methylation activity that is strongly controlled by nutritional cues Schizosaccharomyces pombe