EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.6 | expressed in Escherichia coli BL21(DE3) cells | Peribacillus psychrosaccharolyticus |
EC Number | General Stability | Organism |
---|---|---|
2.4.2.6 | the enzyme is stable when immobilized on PEI-agarose but shows no activity when immobilized on glyoxyl-agarose. The best stability is obtained using dextrane oxidized at 70% (w/v) | Peribacillus psychrosaccharolyticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.6 | NaCl | activity decreases 25% in presence of 1.5 M NaCl | Peribacillus psychrosaccharolyticus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.6 | Na+ | - |
Peribacillus psychrosaccharolyticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | 16000 | - |
6 * 16000, SDS-PAGE | Peribacillus psychrosaccharolyticus |
2.4.2.6 | 91400 | - |
gel filtration | Peribacillus psychrosaccharolyticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.6 | 2-deoxy-D-ribosyl-base1 + base2 | Peribacillus psychrosaccharolyticus | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2.4.2.6 | 2-deoxy-D-ribosyl-base1 + base2 | Peribacillus psychrosaccharolyticus CECT 4074 | - |
2-deoxy-D-ribosyl-base2 + base1 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.6 | Peribacillus psychrosaccharolyticus | - |
- |
- |
2.4.2.6 | Peribacillus psychrosaccharolyticus CECT 4074 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.6 | Bio-Scale Mini UNOsphere Q column chromatography, and Superose 12 gel filtration | Peribacillus psychrosaccharolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.6 | 2'-deoxyuridine + 5-trifluorothymine | - |
Peribacillus psychrosaccharolyticus | 5-trifluorothymidine + uracil | - |
? | |
2.4.2.6 | 2'-deoxyuridine + 5-trifluorothymine | - |
Peribacillus psychrosaccharolyticus CECT 4074 | 5-trifluorothymidine + uracil | - |
? | |
2.4.2.6 | 2'-deoxyuridine + adenine | - |
Peribacillus psychrosaccharolyticus | 2'-deoxyadenosine + uracil | - |
? | |
2.4.2.6 | 2'-deoxyuridine + adenine | - |
Peribacillus psychrosaccharolyticus CECT 4074 | 2'-deoxyadenosine + uracil | - |
? | |
2.4.2.6 | 2'-deoxyuridine + thymidine | - |
Peribacillus psychrosaccharolyticus | 2'-deoxythymidine + uracil | - |
? | |
2.4.2.6 | 2'-deoxyuridine + thymidine | - |
Peribacillus psychrosaccharolyticus CECT 4074 | 2'-deoxythymidine + uracil | - |
? | |
2.4.2.6 | 2-deoxy-D-ribosyl-base1 + base2 | - |
Peribacillus psychrosaccharolyticus | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2.4.2.6 | 2-deoxy-D-ribosyl-base1 + base2 | - |
Peribacillus psychrosaccharolyticus CECT 4074 | 2-deoxy-D-ribosyl-base2 + base1 | - |
? | |
2.4.2.6 | additional information | the enzyme is not able to interchange the deoxyribosyl moiety between ribonucleosides and is free of nucleoside phosphorylases traces | Peribacillus psychrosaccharolyticus | ? | - |
? | |
2.4.2.6 | additional information | the enzyme is not able to interchange the deoxyribosyl moiety between ribonucleosides and is free of nucleoside phosphorylases traces | Peribacillus psychrosaccharolyticus CECT 4074 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.6 | homohexamer | 6 * 16000, SDS-PAGE | Peribacillus psychrosaccharolyticus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.6 | NDT | - |
Peribacillus psychrosaccharolyticus |
2.4.2.6 | nucleoside 2'-deoxyribosyltransferase | - |
Peribacillus psychrosaccharolyticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | 50 | - |
- |
Peribacillus psychrosaccharolyticus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | 4 | 90 | the enzyme is stable up to 50°C during 15 min | Peribacillus psychrosaccharolyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | 8 | - |
- |
Peribacillus psychrosaccharolyticus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.6 | 6 | 10 | the enzyme is stable in a pH range from 6.0 to 9.0, and retains more than 85% activity at pH 10.0 | Peribacillus psychrosaccharolyticus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.4.2.6 | Peribacillus psychrosaccharolyticus | isoelectric focusing | - |
4.6 |