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Literature summary extracted from

  • Zhang, X.; Chen, Y.; Lin, X.; Hong, X.; Zhu, Y.; Li, W.; He, W.; An, F.; Guo, H.
    Adenine phosphoribosyl transferase 1 is a key enzyme catalyzing cytokinin conversion from nucleobases to nucleotides in Arabidopsis (2013), Mol. Plant, 6, 1661-1672.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.7 expressed in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.7 G168E the mutant of isoform APT1 shows reduced activity with zeatin (about 14%) and adenine (about 28%), respectively, compared to the wild type enzyme Arabidopsis thaliana
2.4.2.7 G195D the mutant of isoform APT1 shows nearly no activity with zeatin and adenine, respectively, compared to the wild type enzyme Arabidopsis thaliana
2.4.2.7 G196R the mutant of isoform APT1 shows nearly no activity with zeatin and adenine, respectively, compared to the wild type enzyme Arabidopsis thaliana
2.4.2.7 L96F the mutant of isoform APT1 shows reduced activity with zeatin (about 11%) and adenine (about 8%), respectively, compared to the wild type enzyme Arabidopsis thaliana
2.4.2.7 P85T the mutant of isoform APT1 shows nearly no activity with zeatin and adenine, respectively, compared to the wild type enzyme Arabidopsis thaliana
2.4.2.7 R149K the mutant of isoform APT1 shows no activity with zeatin and adenine, respectively, compared to the wild type enzyme Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Arabidopsis thaliana P31166 ecotype Col-0
-
2.4.2.7 Arabidopsis thaliana Q42563 ecotype Col-0
-
2.4.2.7 Arabidopsis thaliana Q9LFP0 ecotype Col-0
-
2.4.2.7 Arabidopsis thaliana Q9SU38 ecotype Col-0
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.7 Ni affinity column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate isoform APT1 shows 100% activity with adenine Arabidopsis thaliana AMP + diphosphate
-
 ?
2.4.2.7 additional information isoform APT2 is nearly inactive with adenine and zeatin Arabidopsis thaliana ?
-
 ?
2.4.2.7 additional information isoform APT4 is nearly inactive with adenine Arabidopsis thaliana ?
-
 ?
2.4.2.7 additional information isoform APT5 is nearly inactive with adenine Arabidopsis thaliana ?
-
 ?
2.4.2.7 zeatin + 5-phospho-alpha-D-ribose 1-diphosphate isoform APT4 shows about 38% activity with zeatin Arabidopsis thaliana zeatin riboside 5'-phosphate + diphosphate
-
 ?
2.4.2.7 zeatin + 5-phospho-alpha-D-ribose 1-diphosphate isoform APT5 shows 100% activity with zeatin Arabidopsis thaliana zeatin riboside 5'-phosphate + diphosphate
-
 ?
2.4.2.7 zeatin + 5-phospho-alpha-D-ribose 1-diphosphate isoforms APT1 shows 100% activity with zeatin Arabidopsis thaliana zeatin riboside 5'-phosphate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.7 adenine phosphoribosyl transferase
-
 Arabidopsis thaliana
2.4.2.7 APRT
-
 Arabidopsis thaliana
2.4.2.7 APT1 isoform Arabidopsis thaliana
2.4.2.7 APT2 isoform Arabidopsis thaliana
2.4.2.7 APT4 isoform Arabidopsis thaliana
2.4.2.7 APT5 isoform Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.4.2.7 malfunction loss of enzyme activity leads to excess accumulation of cytokinin bases, thus evoking myriad cytokinin-regulated responses, such as delayed leaf senescence, anthocyanin accumulation, and downstream gene expression Arabidopsis thaliana
2.4.2.7 metabolism adenine phosphoribosyl transferase 1 is a key metabolic enzyme participating in the cytokinin inactivation by phosphoribosylation Arabidopsis thaliana