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Literature summary extracted from

  • Naesens, L.; Guddat, L.W.; Keough, D.T.; van Kuilenburg, A.B.; Meijer, J.; Vande Voorde, J.; Balzarini, J.
    Role of human hypoxanthine guanine phosphoribosyltransferase in activation of the antiviral agent T-705 (favipiravir) (2013), Mol. Pharmacol., 84, 615-629.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.8 in complex with T-705-RMP, hanging drop vapor diffusion method, using 0.2 M NaCl, 1 M sodium/potassium tartrate, 0.1 M imidazole, pH 8.0 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 0.0036
-
 hypoxanthine at pH 7.4 and 37°C Homo sapiens
2.4.2.8 0.0054
-
 guanine at pH 7.4 and 37°C Homo sapiens
2.4.2.8 4.069
-
 3-hydroxy-2-pyrazinecarboxamide at pH 7.4 and 37°C Homo sapiens
2.4.2.8 6.426
-
 6-fluoro-3-hydroxy-2-pyrazinecarboxamide at pH 7.4 and 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.8 25000
-
 x * 25000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Homo sapiens P00492
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.8 HEK-293T cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 3-hydroxy-2-pyrazinecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate T-1105, poor substrate Homo sapiens 3-oxo-4-(5-O-phosphono-beta-D-ribofuranosyl)-3,4-dihydropyrazine-2-carboxamide + diphosphate
-
 ?
2.4.2.8 3-hydroxy-2-pyrazinecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate T-1105, poor substrate Homo sapiens ? + diphosphate
-
 ?
2.4.2.8 6-fluoro-3-hydroxy-2-pyrazinecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate T-705, poor substrate Homo sapiens 6-fluoro-3-oxo-4-(5-O-phosphono-beta-D-ribofuranosyl)-3,4-dihydropyrazine-2-carboxamide + diphosphate
-
 ?
2.4.2.8 6-fluoro-3-hydroxy-2-pyrazinecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate T-705, poor substrate Homo sapiens ? + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens IMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.8 ? x * 25000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.2.8 HGPRT
-
 Homo sapiens
2.4.2.8 hypoxanthine guanine phosphoribosyltransferase
-
 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.8 0.027
-
 6-fluoro-3-hydroxy-2-pyrazinecarboxamide at pH 7.4 and 37°C Homo sapiens
2.4.2.8 0.14
-
 3-hydroxy-2-pyrazinecarboxamide at pH 7.4 and 37°C Homo sapiens
2.4.2.8 130
-
 hypoxanthine at pH 7.4 and 37°C Homo sapiens
2.4.2.8 140
-
 guanine at pH 7.4 and 37°C Homo sapiens