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Literature summary extracted from

  • Rubin, E.J.; OBrien, J.P.; Ivanov, P.L.; Brodbelt, J.S.; Trent, M.S.
    Identification of a broad family of lipid A late acyltransferases with non-canonical substrate specificity (2014), Mol. Microbiol., 91, 887-899.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.243 expression in Escherichia coli Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.99.12 lipid A + CMP-beta-Kdo Escherichia coli
-
 alpha-Kdo-(2->6)-lipid A + CMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.243 Helicobacter pylori Q9ZMG5
-
-
2.4.99.12 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.243 a dodecanoyl-[acyl-carrier protein] + (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-dodecanoyloxytetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose
-
 Helicobacter pylori (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-3-O-[(3R)-3-(dodecanoyloxy)tetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + an [acyl-carrier protein]
-
 ?
2.3.1.243 a tetradecanoyl-[acyl-carrier protein] + (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-dodecanoyloxytetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose
-
 Helicobacter pylori (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + an [acyl-carrier protein]
-
 ?
2.3.1.243 additional information enzyme LpxJ catalyzes the addition of a C12:0 or C14:0 acyl chain to the 3'-linked primary acyl chain of lipid IVA. LpxJ can act before the 2' secondary acyltransferase, LpxL, as well as the 3-deoxy-D-manno-octulosonic acid transferase, KdtA. LpxJ can only utilize acyl-ACPs rather than acyl coenzyme A, preferring C12:0-ACP and C14:0-ACP. No substrate: hexa-acylated substrate Kdo2-lipid A Helicobacter pylori ?
-
 ?
2.4.99.12 lipid A + CMP-beta-Kdo
-
 Escherichia coli alpha-Kdo-(2->6)-lipid A + CMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.243 Jhp0255
-
 Helicobacter pylori
2.3.1.243 LpxJ
-
 Helicobacter pylori
2.4.99.12 3-deoxy-D-manno-octulosonic acid transferase
-
 Escherichia coli
2.4.99.12 KdtA
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.3.1.243 physiological function expression of LpxJ complements the defects of an Escherichia coli LpxM mutant Helicobacter pylori