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Literature summary extracted from

  • Buettner, F.F.; Ashikov, A.; Tiemann, B.; Lehle, L.; Bakker, H.
    C. elegans DPY-19 is a C-mannosyltransferase glycosylating thrombospondin repeats (2013), Mol. Cell, 50, 295-302.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.B72 gene dpy-19, recombinant expression in Drosophila melanogaster S2 cells, coexpression with receptor proteins UNC-5 or MIG-21 Caenorhabditis elegans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.B72 endoplasmic reticulum
-
 Caenorhabditis elegans 5783
-
2.4.1.B72 membrane transmembrane protein, membrane topology prediction and spacing between transmembrane domains of DPY-19, overview Caenorhabditis elegans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.B72 Mg2+ required Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.B72 dolichyl D-mannosyl phosphate + MIG-21 protein Caenorhabditis elegans C-mannosylation at a tryptophan residue dolichyl phosphate + C-mannosylated MIG-21 protein
-
 ?
2.4.1.B72 dolichyl D-mannosyl phosphate + UNC-5 protein Caenorhabditis elegans C-mannosylation at a tryptophan residue dolichyl phosphate + C-mannosylated UNC-5 protein
-
 ?
2.4.1.B72 additional information Caenorhabditis elegans cell surface receptors MIG-21 and UNC-5 are acceptor substrates of enzyme DPY-19 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B72 Caenorhabditis elegans P34413 gene dpy-19
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B72 dolichyl D-mannosyl phosphate + MIG-21 protein C-mannosylation at a tryptophan residue Caenorhabditis elegans dolichyl phosphate + C-mannosylated MIG-21 protein
-
 ?
2.4.1.B72 dolichyl D-mannosyl phosphate + UNC-5 protein C-mannosylation at a tryptophan residue Caenorhabditis elegans dolichyl phosphate + C-mannosylated UNC-5 protein
-
 ?
2.4.1.B72 dolichyl D-mannosyl phosphate + WAKW in vitro assay using radiolabeled donor substrate and a synthetic acceptor peptide WAKW, which forms the minimal acceptor substrate Caenorhabditis elegans dolichyl phosphate + ?
-
 ?
2.4.1.B72 additional information cell surface receptors MIG-21 and UNC-5 are acceptor substrates of enzyme DPY-19 Caenorhabditis elegans ?
-
 ?
2.4.1.B72 additional information C-mannosylation of tryptophan residues is an endoplasmic reticulum localized, DPY-19 enzyme-catalyzed reaction using the lipid-linked glycoside dolichol-phosphate-mannose as the donor substrate Caenorhabditis elegans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B72 C-mannosyltransferase
-
 Caenorhabditis elegans
2.4.1.B72 DPY-19
-
 Caenorhabditis elegans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.B72 28
-
 assay at Caenorhabditis elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.B72 7.5
-
 assay at Caenorhabditis elegans

General Information

EC Number General Information Comment Organism
2.4.1.B72 evolution DPY-19 exhibits topological and sequential homology to the N-glycan oligosaccharyltransferase, highlighting an evolutionary link between N- and C-glycosylation. The enzyme is related to the OST family involved in N-glycosylation Caenorhabditis elegans
2.4.1.B72 malfunction identical Q neuroblast migration phenotypes of dpy-19 and mig-21 mutants Caenorhabditis elegans
2.4.1.B72 additional information among the different types of protein glycosylation, C-mannosylation of tryptophan residues stands out because of the unique linkage formed between sugar and protein. Instead of the typical O- or N-glycosidic linkage, a C-C bond is used for attachment of a single mannose. C-mannose is characteristically found in thrombospondin type 1 repeats and in the WSXWS motif of type I cytokine receptors Caenorhabditis elegans
2.4.1.B72 physiological function Caenorhabditis elegans surface receptors MIG-21 and UNC-5 are acceptor substrates of DPY-19, and C-mannosylation is essential for the secretion of soluble MIG-21 Caenorhabditis elegans