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Literature summary extracted from

  • Kadowaki, M.A.; Camilo, C.M.; Muniz, A.B.; Polikarpov, I.
    Functional characterization and low-resolution structure of an endoglucanase Cel45A from the filamentous fungus Neurospora crassa OR74A: thermostable enzyme with high activity toward lichenan and beta-glucan (2015), Mol. Biotechnol., 57, 574-588.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ca2+ 1 mM, 111% of initial activity Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 31200
-
 x * 31200, SDS-PAGE Neurospora crassa

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.4 Acetone 20%, 122% of initial activity Neurospora crassa
3.2.1.4 Ethanol 20%, 119% of initial activity Neurospora crassa
3.2.1.4 isopropanol 20%, 119% of initial activity Neurospora crassa
3.2.1.4 Methanol 20%, 103% of initial activity Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Neurospora crassa Q1K5M0
-
-
3.2.1.4 Neurospora crassa DSM 1257 Q1K5M0
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 0.16
-
 substrate phosphoric acid swollen cellulose, pH 6.0, 60°C Neurospora crassa
3.2.1.4 0.21
-
 substrate carboxymethyl cellulose, pH 6.0, 60°C Neurospora crassa
3.2.1.4 0.54
-
 substrate filter paper , pH 6.0, 60°C Neurospora crassa
3.2.1.4 1.02
-
 substrate beta-glucan, pH 6.0, 60°C Neurospora crassa
3.2.1.4 1.05
-
 substrate lichenan, pH 6.0, 60°C Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 beta-glucan + H2O
-
 Neurospora crassa ?
-
 ?
3.2.1.4 beta-glucan + H2O
-
 Neurospora crassa DSM 1257 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Neurospora crassa ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Neurospora crassa DSM 1257 ?
-
 ?
3.2.1.4 cellopentaose + H2O minimal length substrate Neurospora crassa ?
-
 ?
3.2.1.4 cellopentaose + H2O minimal length substrate Neurospora crassa DSM 1257 ?
-
 ?
3.2.1.4 filter paper + H2O
-
 Neurospora crassa ?
-
 ?
3.2.1.4 lichenan + H2O
-
 Neurospora crassa ?
-
 ?
3.2.1.4 lichenan + H2O
-
 Neurospora crassa DSM 1257 ?
-
 ?
3.2.1.4 additional information enzyme is most active against lichenan and beta-glucans and lesser active toward filter paper, carboxymethyl cellulose, and phosphoric acid-swollen cellulose Neurospora crassa ?
-
 ?
3.2.1.4 additional information enzyme is most active against lichenan and beta-glucans and lesser active toward filter paper, carboxymethyl cellulose, and phosphoric acid-swollen cellulose Neurospora crassa DSM 1257 ?
-
 ?
3.2.1.4 phosphoric acid swollen cellulose + H2O
-
 Neurospora crassa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 31200, SDS-PAGE Neurospora crassa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Cel45A
-
 Neurospora crassa
3.2.1.4 gh45-1
-
 Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60
-
-
 Neurospora crassa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 72
-
 melting temperature Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6
-
-
 Neurospora crassa