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Literature summary extracted from

  • Deves, C.; Rostirolla, D.C.; Martinelli, L.K.; Bizarro, C.V.; Santos, D.S.; Basso, L.A.
    The kinetic mechanism of human thymidine phosphorylase - a molecular target for cancer drug development (2014), Mol. Biosyst., 10, 592-604.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.4 expressed in Escherichia coli Rosetta (DE3) cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.4 KIN59 i.e. 5'-O-tritylinosine, noncompetitive inhibition Homo sapiens
2.4.2.4 thymidine substrate inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.4 0.066
-
 thymidine at pH 7.5 at 37°C Homo sapiens
2.4.2.4 0.107
-
 phosphate at pH 7.5 at 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.4 48855
-
 2 * 48855, mass spectrometry Homo sapiens
2.4.2.4 49024
-
 2 * 49024, calculated from amino acid sequence Homo sapiens
2.4.2.4 50000
-
 2 * 50000, SDS-PAGE Homo sapiens
2.4.2.4 121000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.4 thymidine + phosphate Homo sapiens
-
 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.4 Homo sapiens P19971
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.4 Q-Sepharose column chromatography and phenyl Sepharose column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.4 0.24
-
 crude extract, at pH 7.5 at 37°C Homo sapiens
2.4.2.4 4.3
-
 after 17.9fold purification, at pH 7.5 at 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.4 thymidine + phosphate
-
 Homo sapiens thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.4 homodimer 2 * 50000, SDS-PAGE Homo sapiens
2.4.2.4 homodimer 2 * 48855, mass spectrometry Homo sapiens
2.4.2.4 homodimer 2 * 49024, calculated from amino acid sequence Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.2.4 gliostatin
-
 Homo sapiens
2.4.2.4 PD-ECGF
-
 Homo sapiens
2.4.2.4 platelet derived-endothelial cell growth factor
-
 Homo sapiens
2.4.2.4 TYMP
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.4 7.2
-
 thymidine at pH 7.5 at 37°C Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.2.4 physiological function thymidine phosphorlyase is a key enzyme in the pyrimidine salvage pathway and involved in thymidine homeostasis in cells Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.4 67
-
 phosphate at pH 7.5 at 37°C Homo sapiens
2.4.2.4 109
-
 thymidine at pH 7.5 at 37°C Homo sapiens