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Literature summary extracted from

  • Zhao, N.; Hou, M.; Wang, T.; Chen, Y.; Lv, Y.; Li, Z.; Zhang, R.; Xin, W.; Zou, X.; Hou, L.
    Cloning and expression patterns of the brine shrimp (Artemia sinica) glycogen phosphorylase (GPase) gene during development and in response to temperature stress (2014), Mol. Biol. Rep., 41, 9-18.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.1 GPase gene, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, expression in Escherichia coli strain DH5alpha Artemia sinica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
additional information an allosteric enzyme existing as two interconvertible forms: phosphorylase a (active type) and phosphorylase b (inactive type) Artemia sinica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.1 cytoplasm
-
Artemia sinica 5737
-
2.4.1.1 membrane
-
Artemia sinica 16020
-
2.4.1.1 additional information prediction of protein subcellular localization of the GPase indicates that 52.3% is located in the cytoplasm, 21.7% in the nucleus, 17.4% in the mitochondria and 4.3% in the cytoskeleton and plasma membrane Artemia sinica
-
-
2.4.1.1 nucleus
-
Artemia sinica 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 92500
-
2 * 92500, SDS-PAGE, 2 * 98190, sequence calculation Artemia sinica
2.4.1.1 98190
-
2 * 92500, SDS-PAGE, 2 * 98190, sequence calculation Artemia sinica
2.4.1.1 190000
-
about Artemia sinica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate Artemia sinica
-
[(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Artemia sinica I3QKR5 collected from a salt lake, YunCheng, Shanxi Province, China
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 brain
-
Artemia sinica
-
2.4.1.1 cyst
-
Artemia sinica
-
2.4.1.1 additional information wide distribution of the GPase gene during different developmental stages, expression analysis, overview Artemia sinica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
Artemia sinica [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 homodimer 2 * 92500, SDS-PAGE, 2 * 98190, sequence calculation Artemia sinica

Synonyms

EC Number Synonyms Comment Organism
2.4.1.1 alpha-1,4 glucan phosphorylase UniProt Artemia sinica
2.4.1.1 GPase
-
Artemia sinica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.1 Artemia sinica sequence calcualtion
-
5.67

Expression

EC Number Organism Comment Expression
2.4.1.1 Artemia sinica expression patterns of the glycogen phosphorylase gene during development and in response to temperature stress, overview. Expression is downregulated at temperatures ranging from 20°C to 25°C and is subsequently upregulated in the range 5-15°C additional information

General Information

EC Number General Information Comment Organism
2.4.1.1 metabolism glycogen phosphorylase is a key enzyme involved in intracellular glycogen catabolism, catalyzing the first step in glycogen degradation. In the diapause, GPase catalyzes glycogen into the closely related molecule, sorbitol. The GPase gene expression is stress-related and might play an important role in Artemia development and metabolism Artemia sinica