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Literature summary extracted from

  • Christiansen, I.; Hengstenberg, W.
    Staphylococcal phosphoenolpyruvate-dependent phosphotransferase system - two highly similar glucose permeases in Staphylococcus carnosus with different glucoside specificity: protein engineering in vivo? (1999), Microbiology, 145, 2881-2889.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.199 expressed in Escherichia coli BL21(DE3) cells Staphylococcus carnosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.199 2-deoxy-D-glucose inhibitor of isoform EIICBAGlc1 Staphylococcus carnosus
2.7.1.199 2-Nitrophenyl beta-D-glucoside inhibitor of isoform EIICBAGlc2 Staphylococcus carnosus
2.7.1.199 4-nitrophenyl alpha-D-glucoside inhibitor of isoform EIICBAGlc2 Staphylococcus carnosus
2.7.1.199 methyl alpha-D-glucoside inhibitor of isoform EIICBAGlc2 Staphylococcus carnosus
2.7.1.199 methyl beta-D-glucoside inhibitor of isoforms EIICBAGlc1 and EIICBAGlc2 Staphylococcus carnosus
2.7.1.199 Salicin inhibitor of isoform EIICBAGlc2 Staphylococcus carnosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.199 0.012
-
 D-glucose[side 1] membrane-bound isoform EIICBAGlc1, at pH 7.5 and 37°C Staphylococcus carnosus
2.7.1.199 0.019
-
 D-glucose[side 1] membrane-bound isoform EIICBAGlc2, at pH 7.5 and 37°C Staphylococcus carnosus
2.7.1.199 0.0305
-
 D-glucose[side 1] solubilized isoform EIICBAGlc1, at pH 7.5 and 37°C Staphylococcus carnosus
2.7.1.199 0.0372
-
 D-glucose[side 1] solubilized isoform EIICBAGlc2, at pH 7.5 and 37°C Staphylococcus carnosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.199 membrane
-
 Staphylococcus carnosus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.199 [protein]-Npi-phospho-L-histidine + D-glucose[side 1] Staphylococcus carnosus high specificity for D-glucose [protein]-L-histidine + D-glucose 6-phosphate[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.199 Staphylococcus carnosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.199 Ni2+-NTA column chromatography and Fractogel EMD DMAE Ion-exchange chromatography Staphylococcus carnosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.199 3.125
-
 solubilized isoform EIICBAGlc2 after 381fold purification, at pH 7.5 and 37°C Staphylococcus carnosus
2.7.1.199 6.019
-
 solubilized isoform EIICBAGlc1 after 255fold purification, at pH 7.5 and 37°C Staphylococcus carnosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.199 additional information natural mono- or disaccharides, such as mannose or N-acetylglucosamine are not phosphorylated by either isoform EIICBAGlc1 nor EIICBAGlc2 Staphylococcus carnosus ?
-
 ?
2.7.1.199 [protein]-Npi-phospho-L-histidine + D-glucose[side 1] high specificity for D-glucose Staphylococcus carnosus [protein]-L-histidine + D-glucose 6-phosphate[side 2]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.199 EIICBAGlc1 isoform Staphylococcus carnosus
2.7.1.199 EIICBAGlc2 isoform Staphylococcus carnosus
2.7.1.199 glcA
-
 Staphylococcus carnosus
2.7.1.199 glucose permease
-
 Staphylococcus carnosus
2.7.1.199 PtsG
-
 Staphylococcus carnosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.199 0.0094
-
 methyl beta-D-glucoside solubilized isoform EIICBAGlc1, at pH 7.5 and 37°C Staphylococcus carnosus
2.7.1.199 0.0179
-
 Salicin solubilized isoform EIICBAGlc2, at pH 7.5 and 37°C Staphylococcus carnosus
2.7.1.199 0.0218
-
 methyl beta-D-glucoside solubilized isoform EIICBAGlc2, at pH 7.5 and 37°C Staphylococcus carnosus
2.7.1.199 0.0285
-
 2-Nitrophenyl beta-D-glucoside solubilized isoform EIICBAGlc2, at pH 7.5 and 37°C Staphylococcus carnosus
2.7.1.199 0.0321
-
 4-nitrophenyl alpha-D-glucoside solubilized isoform EIICBAGlc2, at pH 7.5 and 37°C Staphylococcus carnosus
2.7.1.199 0.0349
-
 methyl alpha-D-glucoside solubilized isoform EIICBAGlc2, at pH 7.5 and 37°C Staphylococcus carnosus
2.7.1.199 0.0397
-
 2-deoxy-D-glucose solubilized isoform EIICBAGlc1, at pH 7.5 and 37°C Staphylococcus carnosus