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Literature summary extracted from

  • Garcia-Garcia, J.D.; Girard, L.; Hernandez, G.; Saavedra, E.; Pardo, J.P.; Rodriguez-Zavala, J.S.; Encalada, R.; Reyes-Prieto, A.; Mendoza-Cozatl, D.G.; Moreno-Sanchez, R.
    Zn-bis-glutathionate is the best co-substrate of the monomeric phytochelatin synthase from the photosynthetic heavy metal-hyperaccumulator Euglena gracilis (2014), Metallomics, 6, 604-616.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.15 expressed in Escherichia coli BL21(DE3) cells Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.15 EGTA
-
 Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.15 0.0015
-
 Cd2+-bis-glutathionate in the presence of 0.02 mM GSH, at pH 8.0, temperature not specified in the publication Euglena gracilis
2.3.2.15 0.0016
-
 Zn2+-bis-glutathionate in the presence of 0.02 mM GSH, at pH 8.0, temperature not specified in the publication Euglena gracilis
2.3.2.15 0.0018
-
 Zn2+-bis-glutathionate in the presence of 0.04 mM GSH, at pH 8.0, temperature not specified in the publication Euglena gracilis
2.3.2.15 0.0025
-
 Cd2+-bis-glutathionate in the presence of 0.04 mM GSH, at pH 8.0, temperature not specified in the publication Euglena gracilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.15 Cd2+ required Euglena gracilis
2.3.2.15 Zn2+ required Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.15 Euglena gracilis A0A075TCK5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.15 Talon metal affinity resin column chromatography, and gel filtration Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.15 Cd2+-bis-glutathionate + [Glu(-Cys)]n-Gly
-
 Euglena gracilis ?
-
 ?
2.3.2.15 metal-bis-glutathionate + [Glu(-Cys)]n-Gly Zn2+-bis-glutathionate is the best cosubstrate. The order of preference for the Me-GS2 substrate is Zn2+ > Cd2+ > Mn2+ > As5+ > As3+ > Cu2+ > Ag+ Euglena gracilis ?
-
 ?
2.3.2.15 additional information the enzyme does not use gamma-glutamylcysteine or phytochelatin2 as substrate Euglena gracilis ?
-
 ?
2.3.2.15 Zn2+-bis-glutathionate + [Glu(-Cys)]n-Gly Zn2+-bis-glutathionate is the best cosubstrate Euglena gracilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.15 monomer
-
 Euglena gracilis

Synonyms

EC Number Synonyms Comment Organism
2.3.2.15 PCS
-
 Euglena gracilis
2.3.2.15 phytochelatin synthase
-
 Euglena gracilis

General Information

EC Number General Information Comment Organism
2.3.2.15 physiological function the enzyme confers resistance on Cd2+ stress Euglena gracilis