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Literature summary extracted from

  • Kuo, J.; Khosla, C.
    The initiation ketosynthase (FabH) is the sole rate-limiting enzyme of the fatty acid synthase of Synechococcus sp. PCC 7002 (2014), Metab. Eng., 22, 53-59.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.180 gene fabH, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Synechococcus sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.180 additional information the Synechococcus FabH is used for replacement of its counterpart in the reconstituted Escherichia coli fatty acid synthase system, the resulting complex is strongly limited in FabH activity. The introduction of the Escherichia coli FabH enzyme into the Synechococcus fatty acid synthase system virtually eliminates its dependence on the FabH subunit Synechococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.180 acetyl-CoA + a malonyl-[acyl-carrier protein] Synechococcus sp.
-
 an acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.180 Synechococcus sp. B1XPH5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.180 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and anion exchange chromatography, followed by ultrafiltration Synechococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.180 acetyl-CoA + a malonyl-[acyl-carrier protein]
-
 Synechococcus sp. an acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 additional information Product profile analysis of FabH from Synechococcus sp. strain PCC 7002, overview. t´The major products are lauric acid, myristic acid, palmitic acid, and stearic acid Synechococcus sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.180 FabH
-
 Synechococcus sp.
2.3.1.180 initiation ketosynthase
-
 Synechococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.180 23 25 assay at room temperature Synechococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.180 7.5
-
 assay at Synechococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.180 acetyl-CoA
-
 Synechococcus sp.

General Information

EC Number General Information Comment Organism
2.3.1.180 metabolism the initiation ketosynthase FabH is the sole rate-limiting enzyme of the fatty acid synthesis of Synechococcus sp. PCC 700. Reconstitution of the fatty acid synthase system, FAS. FabH ketosynthase initiates product synthesis by condensing malonyl-ACP with acetyl-CoA to form acetoacetyl-ACP Synechococcus sp.