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Literature summary extracted from

  • Choi, Y.; Tuzikov, A.B.; Ovchinnikova, T.V.; Bovin, N.V.; Pshezhetsky, A.V.
    Novel direct assay for acetyl-CoA:alpha-glucosaminide N-acetyltransferase using BODIPY-glucosamine as a substrate (2015), JIMD Rep., 28, 11-18.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.78 analysis direct method to assay HGSNAT enzymatic activity using fluorescent BODIPY-glucosamine, i.e. 1-[4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl-glycylamino]-beta-D-glucosamine, as a substrate Homo sapiens
2.3.1.78 medicine direct method to assay HGSNAT enzymatic activity using fluorescent BODIPY-glucosamine, i.e. 1-[4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl-glycylamino]-beta-D-glucosamine, as a substrate. Cultured fibroblasts of mucopolysaccharidosis III type C patients show a profound deficiency of HGSNAT activity as compared to normal controls as well as to mucopolysaccharidosis III type A and D patients known to have normal HGSNAT activity Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.78 transfection of COS-7 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.78 D-glucosamine
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.78 Homo sapiens Q68CP4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.78 acetyl-CoA + 1-[4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl-glycylamino]-beta-D-glucosamine i.e. fluorescent BODIPY-glucosamine Homo sapiens CoA + 1-[4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl-glycylamino]-N-acetyl-beta-D-glucosamine
-
 ?
2.3.1.78 acetyl-CoA + 4-methylumbelliferyl-beta-D-glucosaminide
-
 Homo sapiens CoA + 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide
-
 ?
2.3.1.78 acetyl-CoA + heparan sulfate alpha-D-glucosaminide
-
 Homo sapiens CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.78 HGSNAT
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.78 0.26
-
 D-glucosamine substrate 1-[4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl-glycylamino]-beta-D-glucosamine, pH 6.5, 37°C Homo sapiens
2.3.1.78 0.28
-
 D-glucosamine substrate 4-methylumbelliferyl-beta-D-glucosaminide, pH 6.5, 37°C Homo sapiens