EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.2.8 | apo-form and in complex with GMP, sitting drop vapor diffusion method, using 0.2 M trimethylamine N-oxide dehydrate, 0.1 M Tris pH 8.5, 20% (w/v) polyethylene glycol monomethyl ether 2000 | Legionella pneumophila |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.2.8 | S103R | the mutation is associated with Gout arthritis and shows strongly reduced activity compared to the wild type enzyme | Legionella pneumophila |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.8 | 0.0105 | - |
guanine | with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C | Legionella pneumophila | |
2.4.2.8 | 0.06 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C | Legionella pneumophila | |
2.4.2.8 | 0.104 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C | Legionella pneumophila | |
2.4.2.8 | 0.135 | - |
guanine | with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C | Legionella pneumophila |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.8 | GMP + diphosphate | Legionella pneumophila | - |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.4.2.8 | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Legionella pneumophila | - |
GMP + diphosphate | - |
r | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Legionella pneumophila | - |
IMP + diphosphate | - |
r | |
2.4.2.8 | IMP + diphosphate | Legionella pneumophila | - |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.8 | Legionella pneumophila | Q5ZVC1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.8 | Ni-NTA column chromatography and Superdex 200 gel filtration | Legionella pneumophila |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.8 | GMP + diphosphate | - |
Legionella pneumophila | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.4.2.8 | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Legionella pneumophila | GMP + diphosphate | - |
r | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Legionella pneumophila | IMP + diphosphate | - |
r | |
2.4.2.8 | IMP + diphosphate | - |
Legionella pneumophila | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.8 | HGPRT | - |
Legionella pneumophila |
2.4.2.8 | hypoxanthine-guanine phosphoribosyltransferase | - |
Legionella pneumophila |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.8 | 34 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C | Legionella pneumophila | |
2.4.2.8 | 53 | - |
guanine | with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C | Legionella pneumophila | |
2.4.2.8 | 1200 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C | Legionella pneumophila | |
2.4.2.8 | 5100 | - |
guanine | with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C | Legionella pneumophila |