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Literature summary extracted from

  • Zhang, N.; Gong, X.; Lu, M.; Chen, X.; Qin, X.; Ge, H.
    Crystal structures of Apo and GMP bound hypoxanthine-guanine phosphoribosyltransferase from Legionella pneumophila and the implications in gouty arthritis (2016), J. Struct. Biol., 194, 311-316.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.8 apo-form and in complex with GMP, sitting drop vapor diffusion method, using 0.2 M trimethylamine N-oxide dehydrate, 0.1 M Tris pH 8.5, 20% (w/v) polyethylene glycol monomethyl ether 2000 Legionella pneumophila

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.8 S103R the mutation is associated with Gout arthritis and shows strongly reduced activity compared to the wild type enzyme Legionella pneumophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 0.0105
-
guanine with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C Legionella pneumophila
2.4.2.8 0.06
-
5-phospho-alpha-D-ribose 1-diphosphate with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C Legionella pneumophila
2.4.2.8 0.104
-
5-phospho-alpha-D-ribose 1-diphosphate with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C Legionella pneumophila
2.4.2.8 0.135
-
guanine with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C Legionella pneumophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.8 GMP + diphosphate Legionella pneumophila
-
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate Legionella pneumophila
-
GMP + diphosphate
-
r
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Legionella pneumophila
-
IMP + diphosphate
-
r
2.4.2.8 IMP + diphosphate Legionella pneumophila
-
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Legionella pneumophila Q5ZVC1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.8 Ni-NTA column chromatography and Superdex 200 gel filtration Legionella pneumophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 GMP + diphosphate
-
Legionella pneumophila guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Legionella pneumophila GMP + diphosphate
-
r
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Legionella pneumophila IMP + diphosphate
-
r
2.4.2.8 IMP + diphosphate
-
Legionella pneumophila hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Synonyms

EC Number Synonyms Comment Organism
2.4.2.8 HGPRT
-
Legionella pneumophila
2.4.2.8 hypoxanthine-guanine phosphoribosyltransferase
-
Legionella pneumophila

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.8 34
-
5-phospho-alpha-D-ribose 1-diphosphate with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C Legionella pneumophila
2.4.2.8 53
-
guanine with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C Legionella pneumophila
2.4.2.8 1200
-
5-phospho-alpha-D-ribose 1-diphosphate with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C Legionella pneumophila
2.4.2.8 5100
-
guanine with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C Legionella pneumophila