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Literature summary extracted from

  • Patel, H.; Shim, D.; Farinas, E.; Jordan, F.
    Investigation of the donor and acceptor range for chiral carboligation catalyzed by the E1 component of the 2-oxoglutarate dehydrogenase complex (2013), J. Mol. Catal. B, 98, 42-45.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.2.1.5 synthesis application of the E1 component of the Escherichia coli 2-oxoglutarate dehydrogenase multienzyme complex in the synthesis of chiral ompounds with multiple functional groups in good yield and high enantiomeric excess, by varying both the donor substrate (different 2-oxo acids) and the acceptor substrate (glyoxylate, ethyl glyoxylate and methyl glyoxal). The enzyme can accept 2-oxovalerate and 2-oxoisovalerate in addition to its natural substrate 2-oxoglutarate, and the tested acceptors are also acceptable in the carboligation reaction Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.5 Escherichia coli P0AFG3 2-oxoglutarate dehydrogenase E1 component
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.5 2-oxoglutarate + ethyl glyoxylate
-
Escherichia coli (R)-6-ethoxy-5-hydroxy-4,6-dioxohexanoic acid + CO2 81% enantiomeric excess ?
2.2.1.5 2-oxoglutarate + glyoxylate
-
Escherichia coli (R)-2-hydroxy-3-oxoadipate + CO2 90% enantiomeric excess ?
2.2.1.5 2-oxoglutarate + methylglyoxal
-
Escherichia coli (R)-5-hydroxy-4,6-dioxoheptanoic acid + CO2 83% enantiomeric excess ?
2.2.1.5 2-oxoisovalerate + ethyl glyoxylate
-
Escherichia coli (S)-ethyl 2-hydroxy-4-methyl-3-oxopentanoic acid + CO2 81% enantiomeric excess ?
2.2.1.5 2-oxoisovalerate + glyoxylate
-
Escherichia coli (S)-2-hydroxy-4-methyl-3-oxopentanoic acid + CO2 67% enantiomeric excess ?
2.2.1.5 2-oxoisovalerate + methylglyoxal
-
Escherichia coli (S)-3-hydroxy-5-methylhexane-2,4-dione + CO2 more than 90% enantiomeric excess ?
2.2.1.5 2-oxovalerate + ethyl glyoxylate
-
Escherichia coli (S)-ethyl 2-hydroxy-3-oxohexanoic acid + CO2 52% enantiomeric excess ?
2.2.1.5 2-oxovalerate + glyoxylate
-
Escherichia coli (S)-2-hydroxy-3-oxohexanoic acid + CO2 96% enantiomeric excess ?
2.2.1.5 2-oxovalerate + methylglyoxal
-
Escherichia coli (S)-3-hydroxyheptane-2,4-dione + CO2 81% enantiomeric excess ?