EC Number | Application | Comment | Organism |
---|---|---|---|
2.2.1.5 | synthesis | application of the E1 component of the Escherichia coli 2-oxoglutarate dehydrogenase multienzyme complex in the synthesis of chiral ompounds with multiple functional groups in good yield and high enantiomeric excess, by varying both the donor substrate (different 2-oxo acids) and the acceptor substrate (glyoxylate, ethyl glyoxylate and methyl glyoxal). The enzyme can accept 2-oxovalerate and 2-oxoisovalerate in addition to its natural substrate 2-oxoglutarate, and the tested acceptors are also acceptable in the carboligation reaction | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.2.1.5 | Escherichia coli | P0AFG3 | 2-oxoglutarate dehydrogenase E1 component | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.5 | 2-oxoglutarate + ethyl glyoxylate | - |
Escherichia coli | (R)-6-ethoxy-5-hydroxy-4,6-dioxohexanoic acid + CO2 | 81% enantiomeric excess | ? | |
2.2.1.5 | 2-oxoglutarate + glyoxylate | - |
Escherichia coli | (R)-2-hydroxy-3-oxoadipate + CO2 | 90% enantiomeric excess | ? | |
2.2.1.5 | 2-oxoglutarate + methylglyoxal | - |
Escherichia coli | (R)-5-hydroxy-4,6-dioxoheptanoic acid + CO2 | 83% enantiomeric excess | ? | |
2.2.1.5 | 2-oxoisovalerate + ethyl glyoxylate | - |
Escherichia coli | (S)-ethyl 2-hydroxy-4-methyl-3-oxopentanoic acid + CO2 | 81% enantiomeric excess | ? | |
2.2.1.5 | 2-oxoisovalerate + glyoxylate | - |
Escherichia coli | (S)-2-hydroxy-4-methyl-3-oxopentanoic acid + CO2 | 67% enantiomeric excess | ? | |
2.2.1.5 | 2-oxoisovalerate + methylglyoxal | - |
Escherichia coli | (S)-3-hydroxy-5-methylhexane-2,4-dione + CO2 | more than 90% enantiomeric excess | ? | |
2.2.1.5 | 2-oxovalerate + ethyl glyoxylate | - |
Escherichia coli | (S)-ethyl 2-hydroxy-3-oxohexanoic acid + CO2 | 52% enantiomeric excess | ? | |
2.2.1.5 | 2-oxovalerate + glyoxylate | - |
Escherichia coli | (S)-2-hydroxy-3-oxohexanoic acid + CO2 | 96% enantiomeric excess | ? | |
2.2.1.5 | 2-oxovalerate + methylglyoxal | - |
Escherichia coli | (S)-3-hydroxyheptane-2,4-dione + CO2 | 81% enantiomeric excess | ? |