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Literature summary extracted from

  • Inoue, H.; Nishito, A.; Eriguchi, S.; Tamura, T.; Inagaki, K.; Tanaka, H.
    Purification and substrate characterization of alpha-ketobutyrate decarboxylase from Pseudomonas putida (2003), J. Mol. Catal. B, 23, 265-271.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.4.1 expression in Escherichia coli Pseudomonas putida

General Stability

EC Number General Stability Organism
1.2.4.1 purified enzyme is highly sensitive to contaminating proteolytic activity Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.4.1 0.016
-
2-oxobutanoate pH 6.7, 30°C Pseudomonas putida
1.2.4.1 0.147
-
pyruvate pH 6.7, 30°C Pseudomonas putida
1.2.4.1 0.149
-
2-oxovalerate pH 6.7, 30°C Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.4.1 Co2+ or Mg2+, Mn2+, best activating metal ions Pseudomonas putida
1.2.4.1 Mg2+ or Mn2+, Co2+, best activating metal ions Pseudomonas putida
1.2.4.1 Mn2+ or Mg2+, Co2+, best activating metal ions Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.4.1 93000
-
2 * 93000, SDS-PAGE, 2 * 98082, calculated Pseudomonas putida
1.2.4.1 98082
-
2 * 93000, SDS-PAGE, 2 * 98082, calculated Pseudomonas putida
1.2.4.1 185000
-
gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.1 Pseudomonas putida O33468 pyruvate dehydrogenase E1 component
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.4.1 recombinant protein Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.4.1 87
-
pH 6.7, 30°C Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.2.4.1 -20°C, stable for several months Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.4.1 2-oxobutanoate + oxidized 2,6-dichlorophenolindophenol
-
Pseudomonas putida propanal + CO2 + reduced 2,6-dichlorophenolindophenol
-
?
1.2.4.1 2-oxoisovalerate + oxidized 2,6-dichlorophenolindophenol 16% of the activity with 2-oxobutanoate Pseudomonas putida 2-methylpropanal + CO2 + reduced 2,6-dichlorophenolindophenol
-
?
1.2.4.1 2-oxovalerate + oxidized 2,6-dichlorophenolindophenol 49% of the activity with 2-oxobutanoate Pseudomonas putida butanal + CO2 + reduced 2,6-dichlorophenolindophenol
-
?
1.2.4.1 pyruvate + oxidized 2,6-dichlorophenolindophenol 17% of the activity with 2-oxobutanoate Pseudomonas putida acetaldehyde + CO2 + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

EC Number Subunits Comment Organism
1.2.4.1 dimer 2 * 93000, SDS-PAGE, 2 * 98082, calculated Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.2.4.1 MdeB
-
Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.4.1 60
-
-
Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.4.1 43
-
stable up to Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.4.1 0.05
-
pyruvate pH 6.7, 30°C Pseudomonas putida
1.2.4.1 0.128
-
2-oxovalerate pH 6.7, 30°C Pseudomonas putida
1.2.4.1 0.17
-
2-oxobutanoate pH 6.7, 30°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.4.1 6.6
-
-
Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.4.1 thiamine diphosphate Km value 0.017 mM, in presence of Mg2+ and 2-oxobutanoate Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.4.1 0.36
-
pyruvate pH 6.7, 30°C Pseudomonas putida
1.2.4.1 0.86
-
2-oxovalerate pH 6.7, 30°C Pseudomonas putida
1.2.4.1 10.7
-
2-oxobutanoate pH 6.7, 30°C Pseudomonas putida