EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.4.1 | expression in Escherichia coli | Pseudomonas putida |
EC Number | General Stability | Organism |
---|---|---|
1.2.4.1 | purified enzyme is highly sensitive to contaminating proteolytic activity | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.4.1 | 0.016 | - |
2-oxobutanoate | pH 6.7, 30°C | Pseudomonas putida | |
1.2.4.1 | 0.147 | - |
pyruvate | pH 6.7, 30°C | Pseudomonas putida | |
1.2.4.1 | 0.149 | - |
2-oxovalerate | pH 6.7, 30°C | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.4.1 | Co2+ | or Mg2+, Mn2+, best activating metal ions | Pseudomonas putida | |
1.2.4.1 | Mg2+ | or Mn2+, Co2+, best activating metal ions | Pseudomonas putida | |
1.2.4.1 | Mn2+ | or Mg2+, Co2+, best activating metal ions | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.4.1 | 93000 | - |
2 * 93000, SDS-PAGE, 2 * 98082, calculated | Pseudomonas putida |
1.2.4.1 | 98082 | - |
2 * 93000, SDS-PAGE, 2 * 98082, calculated | Pseudomonas putida |
1.2.4.1 | 185000 | - |
gel filtration | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.4.1 | Pseudomonas putida | O33468 | pyruvate dehydrogenase E1 component | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.4.1 | recombinant protein | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.4.1 | 87 | - |
pH 6.7, 30°C | Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.2.4.1 | -20°C, stable for several months | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.4.1 | 2-oxobutanoate + oxidized 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | propanal + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.2.4.1 | 2-oxoisovalerate + oxidized 2,6-dichlorophenolindophenol | 16% of the activity with 2-oxobutanoate | Pseudomonas putida | 2-methylpropanal + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.2.4.1 | 2-oxovalerate + oxidized 2,6-dichlorophenolindophenol | 49% of the activity with 2-oxobutanoate | Pseudomonas putida | butanal + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.2.4.1 | pyruvate + oxidized 2,6-dichlorophenolindophenol | 17% of the activity with 2-oxobutanoate | Pseudomonas putida | acetaldehyde + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.4.1 | dimer | 2 * 93000, SDS-PAGE, 2 * 98082, calculated | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.4.1 | MdeB | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.4.1 | 60 | - |
- |
Pseudomonas putida |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.4.1 | 43 | - |
stable up to | Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.4.1 | 0.05 | - |
pyruvate | pH 6.7, 30°C | Pseudomonas putida | |
1.2.4.1 | 0.128 | - |
2-oxovalerate | pH 6.7, 30°C | Pseudomonas putida | |
1.2.4.1 | 0.17 | - |
2-oxobutanoate | pH 6.7, 30°C | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.4.1 | 6.6 | - |
- |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.4.1 | thiamine diphosphate | Km value 0.017 mM, in presence of Mg2+ and 2-oxobutanoate | Pseudomonas putida |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.4.1 | 0.36 | - |
pyruvate | pH 6.7, 30°C | Pseudomonas putida | |
1.2.4.1 | 0.86 | - |
2-oxovalerate | pH 6.7, 30°C | Pseudomonas putida | |
1.2.4.1 | 10.7 | - |
2-oxobutanoate | pH 6.7, 30°C | Pseudomonas putida |