Literature summary extracted from
Izumi, A.; Rea, D.; Adachi, T.; Unzai, S.; Park, S.Y.; Roper, D.I.; Tame, J.R.
Structure and mechanism of HpcG, a hydratase in the homoprotocatechuate degradation pathway of Escherichia coli (2007), J. Mol. Biol., 370, 899-911.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.2.1.163 |
expressed in Escherichia coli BL21(DE3) and B834(DE3) cells |
Escherichia coli |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.2.1.163 |
hanging drop vapor diffusion method, crystals of the native and selenomethionine-labelled enzyme are grown at 0.1M MES (pH 6.0), 0.7 M potassium thiocyanate, 7% (w/v) PEG 12000, 5% (w/v) PEG 550 MME, 10 mM dithiothreitol and 0.1 M Tris (pH 7.0), 0.7 M potassium thiocyanate, 6.4% (w/v) PEG 20000, 7% (w/v) PEG 550 MME,10 mM dithiothreitol, respectively |
Escherichia coli |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.2.1.163 |
Mg2+ |
required for activity. The enzyme shows a slight preference for Mn2+ over Mg2+ |
Escherichia coli |
|
4.2.1.163 |
Mn2+ |
required for activity. The enzyme shows a slight preference for Mn2+ over Mg2+ |
Escherichia coli |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.2.1.163 |
(4Z)-2-oxohept-4-enedioate + H2O |
Escherichia coli |
- |
(4S)-4-hydroxy-2-oxoheptanedioate |
- |
r |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.1.163 |
Escherichia coli |
Q46982 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.2.1.163 |
Q Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration |
Escherichia coli |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.1.163 |
(4Z)-2-oxohept-4-enedioate + H2O |
- |
Escherichia coli |
(4S)-4-hydroxy-2-oxoheptanedioate |
- |
r |
|
4.2.1.163 |
additional information |
the enzyme cannot process 2-oxo-3-heptene-1,7 dioate |
Escherichia coli |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.2.1.163 |
homopentamer |
2 * 000, calculated from amino acid sequence |
Escherichia coli |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.2.1.163 |
HpcG |
- |
Escherichia coli |