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Literature summary extracted from

  • Izumi, A.; Rea, D.; Adachi, T.; Unzai, S.; Park, S.Y.; Roper, D.I.; Tame, J.R.
    Structure and mechanism of HpcG, a hydratase in the homoprotocatechuate degradation pathway of Escherichia coli (2007), J. Mol. Biol., 370, 899-911.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.163 expressed in Escherichia coli BL21(DE3) and B834(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.163 hanging drop vapor diffusion method, crystals of the native and selenomethionine-labelled enzyme are grown at 0.1M MES (pH 6.0), 0.7 M potassium thiocyanate, 7% (w/v) PEG 12000, 5% (w/v) PEG 550 MME, 10 mM dithiothreitol and 0.1 M Tris (pH 7.0), 0.7 M potassium thiocyanate, 6.4% (w/v) PEG 20000, 7% (w/v) PEG 550 MME,10 mM dithiothreitol, respectively Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.163 Mg2+ required for activity. The enzyme shows a slight preference for Mn2+ over Mg2+ Escherichia coli
4.2.1.163 Mn2+ required for activity. The enzyme shows a slight preference for Mn2+ over Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.163 (4Z)-2-oxohept-4-enedioate + H2O Escherichia coli
-
(4S)-4-hydroxy-2-oxoheptanedioate
-
r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.163 Escherichia coli Q46982
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.163 Q Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.163 (4Z)-2-oxohept-4-enedioate + H2O
-
Escherichia coli (4S)-4-hydroxy-2-oxoheptanedioate
-
r
4.2.1.163 additional information the enzyme cannot process 2-oxo-3-heptene-1,7 dioate Escherichia coli ?
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.163 homopentamer 2 * 000, calculated from amino acid sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.2.1.163 HpcG
-
Escherichia coli