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Literature summary extracted from

  • Dmitryjuk, M.; Dopieralska, M.; Lopienska-Biernat, E.; Fraczek, R.
    Purification and partial biochemical-genetic characterization of trehalose 6-phosphate synthase from muscles of adult female Ascaris suum (2013), J. Helminthol., 87, 212-221.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.15 gene tps1, DNA and amino acid sequence determination and analysis, phylogenetic analysis Ascaris suum
2.4.1.15 gene tps2, DNA and amino acid sequence determination and analysis, phylogenetic analysis Ascaris suum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.15 EDTA 55% inhibition at 10 mM; 55% inhibition at 10 mM Ascaris suum
2.4.1.15 Fe3+ 32% inhibition at 10 mM; 32% inhibition at 10 mM Ascaris suum
2.4.1.15 K+ 50% inhibition at 10 mM; 50% inhibition at 10 mM Ascaris suum
2.4.1.15 Zn2+
-
 Ascaris suum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.15 CaCl2 activates 1.44fold at 10 mM Ascaris suum
2.4.1.15 MgCl2 activates 2fold at 10 mM Ascaris suum
2.4.1.15 NaCl activates at 10 mM Ascaris suum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.15 66000
-
 x * 66000, SDS-PAGE Ascaris suum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.15 Ascaris suum H2BIV4 gene tps1
-
2.4.1.15 Ascaris suum H2BIV5 gene TPS2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.15 native enzyme from female worm muscle 265fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Ascaris suum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.15 muscle
-
 Ascaris suum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.15 150.5
-
 purified native enzyme, pH 4.0, 37°C Ascaris suum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.15 additional information the purified native enzyme is not absolutely substrate-specific, besides glucose 6-phosphate, the enzyme is able to use fructose 6-phosphate as an acceptor of glucose. The enzyme is inactive in the presence of glucose and fructose Ascaris suum ?
-
 ?
2.4.1.15 UDP-glucose + D-glucose 6-phosphate
-
 Ascaris suum UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.15 ? x * 66000, SDS-PAGE Ascaris suum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.15 T6P synthase
-
 Ascaris suum
2.4.1.15 TPS
-
 Ascaris suum
2.4.1.15 trehalose 6-phosphate synthase
-
 Ascaris suum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.15 35
-
-
 Ascaris suum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.15 20 60 activity range, at 40°C, the enzyme demonstrates 87.04% of its maximum activity. A rapid decrease in the enzyme activity occurs at 50°C with 35.27% of maximum activity, completely loss of activity above 60°C Ascaris suum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.15 3.8 4.2
-
 Ascaris suum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.15 3 7 activity range Ascaris suum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.15 Ascaris suum isoelectric focusing
-
 5.4

General Information

EC Number General Information Comment Organism
2.4.1.15 evolution the enzyme belongs to the glucosyltransferase-GTB-type superfamily Ascaris suum