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Literature summary extracted from

  • Zhang, S.; Wang, Y.; Song, X.; Hong, J.; Zhang, Y.; Yao, L.
    Improving Trichoderma reesei Cel7B thermostability by targeting the weak spots (2014), J. Chem. Inf. Model., 54, 2826-2833.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Escherichia coli Trichoderma reesei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.4 molecular dynamics simulation and data analysis protocol to identify the weak spots of Trichoderma reesei Cel7B, through assigning the local melting temperature to individual residue pairs. To test the predicted weak spots, a total of eight disulfide bonds are designed in these regions and all enhance the enzyme thermostability. The increased stability, is negatively correlated with the molecular dynamics-predicted melting temperature Trichoderma reesei

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 G155C/G169C introduction of disulfide bond for stabilization of the catalytic domain, melting temperature 54.9°C, decrease in activity with filter paper and avicel Trichoderma reesei
3.2.1.4 G155C/N182C introduction of disulfide bond for stabilization of the catalytic domain, melting temperature 56.5°C, decrease in activity with filter paper and avicel Trichoderma reesei
3.2.1.4 G155C/N182C/N160C/G183C introduction of disulfide bond for stabilization of the catalytic domain, melting temperature 56.9°C, decrease in activity with filter paper Trichoderma reesei
3.2.1.4 G4C/E73C introduction of disulfide bond for stabilization of the catalytic domain, melting temperature 56.9°C, decrease in activity with filter paper and avicel Trichoderma reesei
3.2.1.4 G4C/F71C introduction of disulfide bond for stabilization of the catalytic domain, melting temperature 56.9°C, decrease in activity with avicel Trichoderma reesei
3.2.1.4 G4C/F71C/G155C/N182C introduction of disulfide bond for stabilization of the catalytic domain, melting temperature 60.7°C, increase in activity with filter paper and avicel Trichoderma reesei
3.2.1.4 G4C/F71C/G155C7N182C/N160C/G183C introduction of disulfide bond for stabilization of the catalytic domain, melting temperature 60.6°C, decrease in activity with filter paper and avicel Trichoderma reesei
3.2.1.4 G4C/F71C/N160C/G183C introduction of disulfide bonds for stabilization of the catalytic domain, melting temperature 60.4°C, decrease in activity with filter paper and avicel Trichoderma reesei
3.2.1.4 G4C/F71C/N160C/G183C/S168T introduction of disulfide bond for stabilization of the catalytic domain, melting temperature 62.8°C, increase in activity with filter paper and avicel Trichoderma reesei
3.2.1.4 G81C/V105C introduction of disulfide bond for stabilization of the catalytic domain, melting temperature 55.9°C, decrease in activity with filter paper and avicel Trichoderma reesei
3.2.1.4 N160C/G183C introduction of disulfide bond for stabilization of the catalytic domain, melting temperature 56.8°C, decrease in activity with filter paper and avicel Trichoderma reesei
3.2.1.4 S213C/A296C introduction of disulfide bond for stabilization of the catalytic domain, melting temperature 55.3°C, decrease in activity with filter paper and avicel Trichoderma reesei
3.2.1.4 Y326C/G343C introduction of disulfide bonds for stabilization of the catalytic domain, melting temperature 57.3°C, increase in activity with filter paper and decrease in activity with avicel Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Trichoderma reesei G0RKH9
-
-
3.2.1.4 Trichoderma reesei QM6a G0RKH9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 avicel + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.4 avicel + H2O
-
 Trichoderma reesei QM6a ?
-
 ?
3.2.1.4 filter paper + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.4 filter paper + H2O
-
 Trichoderma reesei QM6a ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 54.6
-
 wild-type catalytic domain, melting temperature Trichoderma reesei