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Literature summary extracted from

  • Xie, T.; Hou, Y.; Li, D.; Yue, Y.; Qian, S.; Chao, Y.
    Structural basis of a mutant Y195I alpha-cyclodextrin glycosyltransferase with switched product specificity from alpha-cyclodextrin to beta-/gamma-cyclodextrin (2014), J. Biotechnol., 182-183, 92-96.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 mutant Y195I is expressed in Escherichia coli BL21(DE3) cells Paenibacillus macerans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.19 mutant Y195I, hanging drop vapor diffusion method, using 0.2 M sodium acetate trihydrate, 0.1 M Tris hydrochloride pH 9.0, 30% (w/v) polyethylene glycol 4000, at 22°C Paenibacillus macerans

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.19 Y195I the mutation drastically alters the cyclodextrin specificity of the enzyme by switching toward the synthesis of both beta- and gamma-cyclodextrins Paenibacillus macerans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.19 starch + glycosyl acceptor Paenibacillus macerans
-
 cyclodextrins the main product is alpha-cyclodextrin ?
2.4.1.19 starch + glycosyl acceptor Paenibacillus macerans 602-1
-
 cyclodextrins the main product is alpha-cyclodextrin ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Paenibacillus macerans
-
-
-
2.4.1.19 Paenibacillus macerans 602-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 Ni-NTA agarose column chromatography and Sephacryl-100 gel filtration Paenibacillus macerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 starch + glycosyl acceptor
-
 Paenibacillus macerans cyclodextrins the main product is alpha-cyclodextrin ?
2.4.1.19 starch + glycosyl acceptor
-
 Paenibacillus macerans 602-1 cyclodextrins the main product is alpha-cyclodextrin ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 alpha-CGTase
-
 Paenibacillus macerans
2.4.1.19 alpha-cyclodextrin glycosyltransferase
-
 Paenibacillus macerans