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Literature summary extracted from

  • Konishi, J.; Ishii, Y.; Onaka, T.; Ohta, Y.; Suzuki, M.; Maruhashi, K.
    Purification and characterization of dibenzothiophene sulfone monooxygenase and FMN-dependent NADH oxidoreductase from the thermophilic bacterium Paenibacillus sp. strain A11-2 (2000), J. Biosci. Bioeng., 90, 607-613.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.14.22 additional information TdsA shows higher activity toward bulkier substrates than its mesophilic counterpart, DszA. These properties suggest the applicability of biodesulfmizatfon to the processing of actual petroleum fractions Paenibacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.22 1,10-phenanthroline
-
 Paenibacillus sp.
1.14.14.22 4-chloromercuribenzoic acid
-
 Paenibacillus sp.
1.14.14.22 5,5'-dithio-bis-2-nitrobenzoic acid
-
 Paenibacillus sp.
1.14.14.22 Ca2+
-
 Paenibacillus sp.
1.14.14.22 Co2+
-
 Paenibacillus sp.
1.14.14.22 Cu2+ strong inhibition Paenibacillus sp.
1.14.14.22 EDTA
-
 Paenibacillus sp.
1.14.14.22 iodoacetic acid
-
 Paenibacillus sp.
1.14.14.22 Mn2+
-
 Paenibacillus sp.
1.14.14.22 additional information no inhibition by 2-hydroxybiphenyl Paenibacillus sp.
1.14.14.22 N-ethylmaleimide
-
 Paenibacillus sp.
1.14.14.22 Na2SO4
-
 Paenibacillus sp.
1.14.14.22 sulfate slightly inhibiting Paenibacillus sp.
1.14.14.22 Zn2+
-
 Paenibacillus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.22 Mg2+ slight activation Paenibacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.22 48000
-
 2 * 48000, SDS-PAGE Paenibacillus sp.
1.14.14.22 120000
-
 gel filtration Paenibacillus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.22 dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Paenibacillus sp.
-
 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?
1.14.14.22 dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Paenibacillus sp. A11-2
-
 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.22 Paenibacillus sp. Q9LBX4 gene tdsA
-
1.14.14.22 Paenibacillus sp. A11-2 Q9LBX4 gene tdsA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.22 native enzyme 47.6fold by anion exchange and hydrophobic interaction chromatography, followed by another different step of anion exchange chromatography and hydroxyapatite chromatography Paenibacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.22 6
-
 purified enzyme, pH 7.0, 50°C, substrate 3,6-dimethyldibenzothiophene-5,5-dioxide Paenibacillus sp.
1.14.14.22 12.4
-
 purified enzyme, pH 7.0, 50°C, substrate 4-methyldibenzothiophene-5,5-dioxide Paenibacillus sp.
1.14.14.22 22.4
-
 purified enzyme, pH 7.0, 50°C, substrate 1-methyldibenzothiophene-5,5-dioxide Paenibacillus sp.
1.14.14.22 29.6
-
 purified enzyme, pH 7.0, 50°C, substrate 3-methyldibenzothiophene-5,5-dioxide Paenibacillus sp.
1.14.14.22 37.2 40 purified enzyme, pH 7.0, 50°C, substrate dibenzothiophene-5,5-dioxide Paenibacillus sp.
1.14.14.22 38.4
-
 purified enzyme, pH 7.0, 50°C, substrate 2-methyldibenzothiophene-5,5-dioxide Paenibacillus sp.
1.14.14.22 62.4
-
 purified enzyme, pH 7.0, 50°C, substrate 3,4,6-trimethyldibenzothiophene-5,5-dioxide Paenibacillus sp.
1.14.14.22 77.2
-
 purified enzyme, pH 7.0, 50°C, substrate 4,6-dimethyldibenzothiophene-5,5-dioxide Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.22 1-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Paenibacillus sp. ?
-
 ?
1.14.14.22 1-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Paenibacillus sp. A11-2 ?
-
 ?
1.14.14.22 2-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Paenibacillus sp. ?
-
 ?
1.14.14.22 2-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Paenibacillus sp. A11-2 ?
-
 ?
1.14.14.22 3,4,6-trimethyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Paenibacillus sp. ?
-
 ?
1.14.14.22 3,4,6-trimethyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Paenibacillus sp. A11-2 ?
-
 ?
1.14.14.22 3,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Paenibacillus sp. ?
-
 ?
1.14.14.22 3,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Paenibacillus sp. A11-2 ?
-
 ?
1.14.14.22 3-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Paenibacillus sp. ?
-
 ?
1.14.14.22 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Paenibacillus sp. ?
-
 ?
1.14.14.22 4-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Paenibacillus sp. ?
-
 ?
1.14.14.22 dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Paenibacillus sp. 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?
1.14.14.22 dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Paenibacillus sp. A11-2 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.22 homodimer 2 * 48000, SDS-PAGE Paenibacillus sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.14.22 DBT sulfone monooxygenase
-
 Paenibacillus sp.
1.14.14.22 dibenzothiophene sulfone monooxygenase
-
 Paenibacillus sp.
1.14.14.22 TdsA
-
 Paenibacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.22 45
-
-
 Paenibacillus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.14.22 30 65 activity range, profile overview Paenibacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.22 60
-
 purified enzyme, pH 7.0, highly stable up to Paenibacillus sp.
1.14.14.22 70
-
 purified enzyme, pH 7.0, inactivation Paenibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.22 5.5
-
-
 Paenibacillus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.22 FMNH2
-
 Paenibacillus sp.
1.14.14.22 additional information the purified TdsA absolutely requires an oxidoreductase for its activity, oxidoreductase TdsD is completely FMN-dependent, and FAD does not act as a cofactor. TsdD is active with NADH and NADPH to the same extent. TdsA exhibits no activity in the absence of TdsD Paenibacillus sp.