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Literature summary extracted from
- Purification and characterization of dibenzothiophene (DBT) sulfone monooxygenase, an enzyme involved in DBT desulfurization, from Rhodococcus erythropolis D-1 (1999), J. Biosci. Bioeng., 88, 610-616.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.22 | gene dszA, DNA and amino acid sequence determination and analysis, sequence comparisons | Rhodococcus erythropolis |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.14.14.22 | purified enzyme, 4°C, hanging drop vapor diffusion method, mixing of 42 mg/ml protein in 1 mM Tris-HCl buffer, pH 8.0, with an equal volume of well solution containing 12% PEG 400, 0.2 M CaCl*, and 0.01 M HEPES buffer, pH 7.5, 1 week, X-ray diffraction structure determination and analysis | Rhodococcus erythropolis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.22 | 1,10-phenanthroline | 37% inhibition at 1 mM | Rhodococcus erythropolis |  |
| 1.14.14.22 | 2,2'-bipyridine | - |
Rhodococcus erythropolis | |
| 1.14.14.22 | 2,2-bipyridine | 62% inhibition at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | 8-Quinolinol | 88% inhibition at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | Ca2+ | 81% inhibition at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | Co2+ | 27% inhibition at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | Cu2+ | complete inhibition at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | EDTA | 49% inhibition at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | Fe2+ | 32% inhibition at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | Hg2+ | complete inhibition at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | Li+ | 20% inhibition at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | Mn2+ | 72% inhibition at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | N-bromosuccinimide | 17% inhibition at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | N-ethylmaleimide | 27% inhibition at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | Ni2+ | 78% inhibition at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | NO3- | 39% inhibition at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | p-chloromercuribenzoic acid | 50% inhibition at 1 mM | Rhodococcus erythropolis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.22 | 5,5'-dithiobis(2-nitrobenzoic acid) | slightly activating at 0.1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | Cd2+ | slightly activating at 1 mM | Rhodococcus erythropolis | |
| 1.14.14.22 | additional information | no or poor effect by 1 mM of Al3+, Zn2+, Mg2+, semicarbazide, and NaF | Rhodococcus erythropolis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.22 | 50000 | - |
2 * 50000, SDS-PAGE | Rhodococcus erythropolis |
| 1.14.14.22 | 97000 | - |
gel filtration | Rhodococcus erythropolis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis D-1 | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.22 | Rhodococcus erythropolis | Q6WE15 | gene dszA | - |
| 1.14.14.22 | Rhodococcus erythropolis D-1 | Q6WE15 | gene dszA | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.22 | native enzyme 102fold to homogeneity by two different steps of anion exchange chromatography, followed by hydrophobic interaction chromatography, and gel filtration | Rhodococcus erythropolis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.22 | 807 | - |
purified enzyme, pH 7.0, 35°C | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis D-1 | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenz[c,e][1,2] oxathiin 6-oxide + 2 FMNH2 + O2 | i.e. sultine, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenz[c,e][1,2] oxathiin 6-oxide + 2 FMNH2 + O2 | i.e. sultine, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis D-1 | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenz[c,e][1,2]oxathiin 6,6-dioxide + 2 FMNH2 + O2 | i.e. sultone, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | dibenz[c,e][1,2]oxathiin 6,6-dioxide + 2 FMNH2 + O2 | i.e. sultone, sultine is nonenzymatically hydrolyzed to form 2'-hydroxybiphenyl 2-sulfinic acid, it is also oxidized to sultone. Once sultone is nonenzymatically formed from sultine, it is immediately converted to 2,2'-dihydroxybiphenyl by DszA | Rhodococcus erythropolis D-1 | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
| 1.14.14.22 | additional information | no activity with dibenzothiophene and 2'-hydroxybiphenyl 2-sulfinic acid, substrate specificity, overview. DszA may recognize the sulfone moiety within the structure of DBT sulfone and sultone | Rhodococcus erythropolis | ? | - |
? | |
| 1.14.14.22 | additional information | no activity with dibenzothiophene and 2'-hydroxybiphenyl 2-sulfinic acid, substrate specificity, overview. DszA may recognize the sulfone moiety within the structure of DBT sulfone and sultone | Rhodococcus erythropolis D-1 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.22 | homodimer | 2 * 50000, SDS-PAGE | Rhodococcus erythropolis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.22 | DBT sulfone monooxygenase | - |
Rhodococcus erythropolis |
| 1.14.14.22 | dszA | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.22 | 35 | - |
- |
Rhodococcus erythropolis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.22 | 35 | - |
purified enzyme, stable up to | Rhodococcus erythropolis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.22 | 7.5 | - |
- |
Rhodococcus erythropolis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.22 | 6 | 10 | purified enzyme, stable at | Rhodococcus erythropolis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.22 | FMNH2 | essentially required | Rhodococcus erythropolis | |
| 1.14.14.22 | additional information | NADH flavin reductase, essentially required | Rhodococcus erythropolis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.22 | metabolism | enzyme DszA is involved in the microbial DBT desulfurization metabolism and catalyzes the conversion of dibenzothiophene sulfone to 2'-hydroxybiphenyl 2-sulfinic acid in the presence of flavin reductase with cleavage of the carbon-sulfur bond in the dibenzothiophene skeleton | Rhodococcus erythropolis |
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