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Literature summary extracted from

  • Fujiwara, S.; Kawazoe, T.; Ohnishi, K.; Kitagawa, T.; Popa, C.; Valls, M.; Genin, S.; Nakamura, K.; Kuramitsu, Y.; Tanaka, N.; Tabuchi, M.
    RipAY, a plant pathogen effector protein exhibits robust gamma-glutamyl cyclotransferase activity when stimulated by eukaryotic thioredoxins (2016), J. Biol. Chem., 291, 6813-6830.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.2.9 thioredoxin the enzyme is activated by 0.008 mM thioredoxin Ralstonia solanacearum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.9 expressed in Escherichia coli Rosetta gami B (DE3) cells Ralstonia solanacearum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.9 2.11
-
 glutathione at pH 8.0 and 37°C Ralstonia solanacearum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.9 50000
-
 x * 50000, SDS-PAGE Ralstonia solanacearum

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.9 Ralstonia solanacearum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.9 Ni-NTA affinity column chromatography Ralstonia solanacearum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.9 glutathione
-
 Ralstonia solanacearum 5-oxoproline + L-Cys-Gly
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.2.9 ? x * 50000, SDS-PAGE Ralstonia solanacearum

Synonyms

EC Number Synonyms Comment Organism
4.3.2.9 gamma-glutamyl cyclotransferase
-
 Ralstonia solanacearum
4.3.2.9 GGCT
-
 Ralstonia solanacearum
4.3.2.9 RipAY
-
 Ralstonia solanacearum
4.3.2.9 RSp1022
-
 Ralstonia solanacearum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.2.9 52.8
-
 glutathione at pH 8.0 and 37°C Ralstonia solanacearum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.2.9 25.2
-
 glutathione at pH 8.0 and 37°C Ralstonia solanacearum