Literature summary extracted from
Drees, S.L.; Li, C.; Prasetya, F.; Saleem, M.; Dreveny, I.; Williams, P.; Hennecke, U.; Emsley, J.; Fetzner, S.
PqsBC, a condensing enzyme in the biosynthesis of the Pseudomonas aeruginosa quinolone signal: crystal structure, inhibition, and reaction mechanism (2016), J. Biol. Chem., 291, 6610-6624.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.3.1.B38 |
- |
Pseudomonas aeruginosa |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.3.1.B38 |
mutant C129A, to 2 A resolution, space group P212121. The heterodimer displays a pseudo 2fold symmetry. Subunit PqsC has an active site comprised of Cys129 and His269 |
Pseudomonas aeruginosa |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.3.1.B38 |
C129A |
mutation in catalytic dyad, crystallization data |
Pseudomonas aeruginosa |
2.3.1.B38 |
V299N |
substitution leads to significant activity toward the deamino substrate analog benzoylacetate, suggesting that the substrate 2-aminobenzoylacetate itself supplies the asparagine-equivalent amino function that assists in catalysis |
Pseudomonas aeruginosa |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.3.1.B38 |
2-aminoacetophenone |
pathway-inherent competitive inhibitor. EC50 of 46 microM in the in vitro assay |
Pseudomonas aeruginosa |
|
2.3.1.B38 |
additional information |
2-heptyl-4(1H)-quinolone and 2-heptyl-3-hydroxy-4(1H)-quinolone as well as ,4-dihydroxyquinoline at concentrations up to 50 microM do not affect PqsBC activity |
Pseudomonas aeruginosa |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.3.1.B38 |
0.006 |
- |
octanoyl-CoA |
pH 8.2, temperature not specified in the publication |
Pseudomonas aeruginosa |
|
2.3.1.B38 |
0.105 |
- |
2-aminobenzoylacetate |
pH 8.2, temperature not specified in the publication |
Pseudomonas aeruginosa |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.3.1.B38 |
additional information |
Pseudomonas aeruginosa |
the catalytic His269 residue is not involved in acyl-enzyme formation, but contributes to an acylation-dependent increase in affinity for the second substrate 2-aminobenzoylacetate. Octanoylation of Cys129 of PqsC is independent of His269 |
? |
- |
? |
|
2.3.1.B38 |
octanoyl-CoA + 2-aminobenzoylacetate |
Pseudomonas aeruginosa |
- |
CoA + 2-heptylquinolin-4(1H)-one + CO2 + H2O |
product is a signal molecule in Pseudomonas aeruginosa. The initial product is 1-(2-aminophenyl)decane-1,3-dione which spontaneously rearranges to the product under the elimination of water |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.1.B38 |
Pseudomonas aeruginosa |
Q9I4X1 |
subunit PqsC |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.3.1.B38 |
additional information |
the catalytic His269 residue is not involved in acyl-enzyme formation, but contributes to an acylation-dependent increase in affinity for the second substrate 2-aminobenzoylacetate. Octanoylation of Cys129 of PqsC is independent of His269 |
Pseudomonas aeruginosa |
? |
- |
? |
|
2.3.1.B38 |
octanoyl-CoA + 2-aminobenzoylacetate |
- |
Pseudomonas aeruginosa |
CoA + 2-heptylquinolin-4(1H)-one + CO2 + H2O |
product is a signal molecule in Pseudomonas aeruginosa. The initial product is 1-(2-aminophenyl)decane-1,3-dione which spontaneously rearranges to the product under the elimination of water |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.3.1.B38 |
PqsBC |
- |
Pseudomonas aeruginosa |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
2.3.1.B38 |
6.8 |
- |
octanoyl-CoA |
pH 8.2, temperature not specified in the publication |
Pseudomonas aeruginosa |
|
Ki Value [mM]
EC Number |
Ki Value [mM] |
Ki Value maximum [mM] |
Inhibitor |
Comment |
Organism |
Structure |
---|
2.3.1.B38 |
0.054 |
0.056 |
2-aminoacetophenone |
pH 8.2, temperature not specified in the publication |
Pseudomonas aeruginosa |
|